Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Screening of Entomopathogenic Fungal Culture Metabolites With Honey Bee Nosemosis Inhibitory Activity

Version 1 : Received: 26 April 2023 / Approved: 27 April 2023 / Online: 27 April 2023 (04:43:22 CEST)

A peer-reviewed article of this Preprint also exists.

Kim, D.-J.; Woo, R.-M.; Kim, K.-S.; Woo, S.-D. Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity. Insects 2023, 14, 538. Kim, D.-J.; Woo, R.-M.; Kim, K.-S.; Woo, S.-D. Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity. Insects 2023, 14, 538.

Abstract

This study aimed to select the most effective metabolites for controlling honey bee nosemosis using culture extracts from 342 entomopathogenic fungi of 24 species from 18 genera. The germination inhibitory activity of the fungal culture extract against Nosema ceranae spores was evaluated using an in vitro germination assay method. Among 89 fungal culture extracts showing germination inhibitory activity of approximately 80% or more, 44 culture extracts that maintained their inhibitory activity even at a concentration of 1% were selected. Finally, the honey bee nosemosis inhibitory activity was evaluated using the cultured extracts of 5 fungal isolates having a Nosema inhibitory activity of approximately 60% or more even when the extract was removed after treatment. As a result, the proliferation of Nosema spores was reduced by all fungal culture extract treatments. However, only the treatment of the culture extracts from Paecilomyces marquandii 364 and Pochonia bulbillosa 60 showed a reduction in honey bee mortality due to nosemosis. In particular, the extracts of these two fungal isolates also increased the survival of honey bees.

Keywords

honey bee; nosemosis; Nosema ceranae; Apis mellifera; entomopathogenic fungi; metabolite

Subject

Biology and Life Sciences, Insect Science

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