Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The YBR056W-A and Its Ortholog YDR034W-B of S. cerevisiae Belonging to CYSTM Family Participate in Manganese Stress Overcoming

Anton Zvonarev
ORCID logo ,
Larisa Ledova
,
Lubov Ryazanova
,
Airat Valiakhmetov
,
Vasilina Farofonova
ORCID logo ,
Tatiana Kulakovskaya
*
Version 1 : Received: 23 April 2023 / Approved: 26 April 2023 / Online: 26 April 2023 (11:47:06 CEST)

A peer-reviewed article of this Preprint also exists.

Zvonarev, A.; Ledova, L.; Ryazanova, L.; Valiakhmetov, A.; Farofonova, V.; Kulakovskaya, T. The YBR056W-A and Its Ortholog YDR034W-B of S. cerevisiae Belonging to CYSTM Family Participate in Manganese Stress Overcoming. Genes 2023, 14, 987. Zvonarev, A.; Ledova, L.; Ryazanova, L.; Valiakhmetov, A.; Farofonova, V.; Kulakovskaya, T. The YBR056W-A and Its Ortholog YDR034W-B of S. cerevisiae Belonging to CYSTM Family Participate in Manganese Stress Overcoming. Genes 2023, 14, 987.

Abstract

The CYSTM (cysteine-rich transmembrane module) protein family comprises small molecular cysteine-rich tail-anchored membrane proteins found in many eukaryotes. The Saccharomyces cerevisiae strains carrying the CYSTM genes YDRO34W-B and YBR056W-A (MNC1) fused with GFP were used to test expression of these genes under different stresses. The YBR056W-A (MNC1) and YDR034W-B genes are expressed under stress conditions caused by the toxic concentrations of heavy metal ions such as manganese, cobalt, nickel, zinc, cuprum, and 2.4-dinitrophenol uncoupler. YDR034W-B but not YBR056W-A is expressed under alkali and cadmium stresses. YBR056W-A is localized in intracellular membranes, while YDR034W-B is localized in the cytoplasmic membrane. The null-mutants in both genes demonstrated both decreased cell concentration and lytic phenotype when cultivated in the presence of excess manganese. This indicates the involvement of Mnc1 and YDR034W-B proteins in manganese detoxification, probably by complexation of its ion.

Keywords

CYSTM; manganese; stress; MNC1; YDR034W-B; yeast

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.