Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Transcriptome Metabolic Characterization of Tuber borchii SP1, a New Spanish Strain for In Vitro Studies of the Bianchetto Truffle

Emilia Chuina Tomazeli
ORCID logo ,
Manuel Alfaro
,
Alessandra Zambonelli
ORCID logo ,
Edurne Garde
,
Gumer Pérez
ORCID logo ,
Lucía Ramírez
,
Hesham Salman
ORCID logo ,
Antonio G. Pisabarro
*
Version 1 : Received: 22 April 2023 / Approved: 23 April 2023 / Online: 23 April 2023 (08:21:55 CEST)

A peer-reviewed article of this Preprint also exists.

Chuina Tomazeli, E.; Alfaro, M.; Zambonelli, A.; Garde, E.; Pérez, G.; Jiménez, I.; Ramírez, L.; Salman, H.; Pisabarro, A.G. Transcriptome Metabolic Characterization of Tuber borchii SP1—A New Spanish Strain for In Vitro Studies of the Bianchetto Truffle. Int. J. Mol. Sci. 2023, 24, 10981. Chuina Tomazeli, E.; Alfaro, M.; Zambonelli, A.; Garde, E.; Pérez, G.; Jiménez, I.; Ramírez, L.; Salman, H.; Pisabarro, A.G. Transcriptome Metabolic Characterization of Tuber borchii SP1—A New Spanish Strain for In Vitro Studies of the Bianchetto Truffle. Int. J. Mol. Sci. 2023, 24, 10981.

Abstract

Truffles are ascomycete hypogeous fungi belonging to the Tuberaceae family of the Pezizales order that grow in ectomycorrhizal symbiosis with tree roots and are known for their peculiar aromas and flavors. Axenic culture of truffle mycelium is problematic because it is not possible in many cases, and the growth rate is meager when it is possible. This limitation prompts searching and characterizing new strains that can be handled in laboratory conditions for basic and applied studies. In this work, a new strain of Tuber borchii (strain SP1) has been isolated and cultured, and its transcriptome has been analyzed under different in vitro culture conditions. The results show that the best T. borchii SP1 growth was obtained using maltose-enriched cultures made with soft-agar and in static submerged cultures made at 22ºC. The transcriptome analysis of this strain cultured in different media indicated that most of the gene transcription effort is due to a limited number of genes (20% of genes account for 80% of the transcription), that the transcription profile of the central metabolism genes was similar in the different conditions analyzed with a transcription signal detected for around 80% of the annotated genes. The gene expression profile suggests that T. borchii uses a fermentative rather than respiratory metabolism, even in aerobic conditions. Finally, there is a reduced expression of genes belonging to secondary metabolite clusters, whereas there is a significative transcription of those involved in producing volatile aromatic compounds.

Keywords

Axenic culture; Aroma genes; Central metabolism; Fermentation; Morphological characterization; Secondary metabolite clusters; Strain isolation; Transcriptome profile; Truffle cultivation; Vegetative mycelium

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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