Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Functional Activity of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes on a Mouse Renal Subcapsular Xenograft Model

Elena Chepeleva
* ORCID logo ,
Sophia Pavlova
ORCID logo ,
Nataliya Bgatova
,
Alexander Volkov
,
Galina Kazanskaya
,
David Sergeevichev
Version 1 : Received: 20 April 2023 / Approved: 21 April 2023 / Online: 21 April 2023 (08:10:14 CEST)

A peer-reviewed article of this Preprint also exists.

Chepeleva, E.V.; Pavlova, S.V.; Bgatova, N.P.; Volkov, A.M.; Kazanskaya, G.M.; Sergeevichev, D.S. Functional Activity of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes on a Mouse Renal Subcapsular Xenograft Model. International Journal of Molecular Sciences 2023, 24, 9792, doi:10.3390/ijms24129792. Chepeleva, E.V.; Pavlova, S.V.; Bgatova, N.P.; Volkov, A.M.; Kazanskaya, G.M.; Sergeevichev, D.S. Functional Activity of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes on a Mouse Renal Subcapsular Xenograft Model. International Journal of Molecular Sciences 2023, 24, 9792, doi:10.3390/ijms24129792.

Abstract

For the replacement of lost contractile elements in the treatment of coronary heart disease, the most promising approaches to obtain cardiomyocytes by cardiac differentiation of pluripotent cells. The aim of this work is to develop a technology for the formation of a functional layer of cardiomyocytes differentiated from iPSCs, capable of generating rhythmic activity and synchronous contractions. To accelerate cardiomyocyte maturation, the renal subcapsular transplantation model was used in SCID mice. After explantation, the formation of cardiomyocyte contractile apparatus was assessed by fluorescence and electron microscopy, and calcium ion oscillation in the cytoplasm was assessed by visualization of the fluorescent calcium binding dye Fluo-8. It was shown that human iPSC-derived cardiomyocyte cell layers transplanted (for up to 6 weeks) under SCID mouse kidney fibrous capsules begin to form an ordered contractile apparatus and retain functional activity and the ability to oscillate calcium ion fluxes after explantation from the body.

Keywords

cardiomyocytes; induced pluripotent stem cells; cell therapy; calcium imaging; fluorescence microscopy; transmission electron microscopy

Subject

Biology and Life Sciences, Cell and Developmental Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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