Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Novel Bioanalytical HPLC-MS/MS Method for Deucravacitinib Determination in Human Plasma

Version 1 : Received: 17 April 2023 / Approved: 18 April 2023 / Online: 18 April 2023 (02:51:35 CEST)

How to cite: Mahesh, P.; Haque, M.A.; Salman, B.I.; Belal, T.S.; Ibrahim, A.E.; El Deeb, S. A Novel Bioanalytical HPLC-MS/MS Method for Deucravacitinib Determination in Human Plasma. Preprints 2023, 2023040459. https://doi.org/10.20944/preprints202304.0459.v1 Mahesh, P.; Haque, M.A.; Salman, B.I.; Belal, T.S.; Ibrahim, A.E.; El Deeb, S. A Novel Bioanalytical HPLC-MS/MS Method for Deucravacitinib Determination in Human Plasma. Preprints 2023, 2023040459. https://doi.org/10.20944/preprints202304.0459.v1

Abstract

Plaque psoriasis is a common, long-lasting illness that affects the immune system and causes significant negative impacts on a patient's physical health, well-being, and ability to work effectively. Deucravacitinib (DEU) is the first oral medication used in the treatment of plaque psoriasis, a chronic skin condition that causes red, scaly patches on the skin. DEU is a type of medication called an oral Janus kinase (JAK) inhibitor, which works by blocking specific enzymes that play a role in the inflammation and immune response associated with psoriasis. Therefore, a quick, easy, novel, reliable, sensitive, and straightforward Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) approach was used to analyse DEU in plasma samples. The LC-MS/MS method for the determination of DEU in human plasma was based on using trimethoprim as an internal standard (IS). The separation of DEU and IS was carried out via liquid-liquid extraction (LLE). The isolated substances were then subjected to the chromatographic system using the ACE-C18 column (4.6x100 mm, 5 µm). The mobile phase employed consisted of methanol and a solution of 2 mM ammonium formate (80:20 v/v, respectively). The flow rate used was set at 0.9 mL min-1. The creative strategy was performed by running an ABSCIEX API 4000 mass spectrometer with an electron spray ionization source in Multiple Reaction Monitoring (MRM) modes. The ion transitions m/z 426.3  358.2 was used for DEU quantitation, while the ion transitions m/z 291.1  261.1 was used for trimethoprim quantitation. The accuracy, precision, linearity, recovery, and selectivity of DEU were deemed acceptable when validated for a concentration range between 0.500 to 601.050 ng/mL, utilizing a weighting factor of 1/x2.

Keywords

Deucravacitinib; HPLC-MS/MS; Psoriasis; Human plasma

Subject

Chemistry and Materials Science, Analytical Chemistry

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.