Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

GmUFO1 Regulates Floral Organ Number and Shape in Soybean

Huimin Yu
,
Yaohua Zhang
* ORCID logo ,
Junling Fang
,
Xinjing Yang
,
Zhirui Zhang
,
Fawei Wang
,
Tao Wu
ORCID logo ,
Muhammad Hafeez Ullah Khan
,
Javaid Akhter Bhat
,
Yu Jiang
,
Yi Wang
,
Xianzhong Feng
* ORCID logo
Version 1 : Received: 10 April 2023 / Approved: 11 April 2023 / Online: 11 April 2023 (04:10:00 CEST)

A peer-reviewed article of this Preprint also exists.

Yu, H.; Zhang, Y.; Fang, J.; Yang, X.; Zhang, Z.; Wang, F.; Wu, T.; Khan, M.H.U.; Bhat, J.A.; Jiang, Y.; Wang, Y.; Feng, X. GmUFO1 Regulates Floral Organ Number and Shape in Soybean. Int. J. Mol. Sci. 2023, 24, 9662. Yu, H.; Zhang, Y.; Fang, J.; Yang, X.; Zhang, Z.; Wang, F.; Wu, T.; Khan, M.H.U.; Bhat, J.A.; Jiang, Y.; Wang, Y.; Feng, X. GmUFO1 Regulates Floral Organ Number and Shape in Soybean. Int. J. Mol. Sci. 2023, 24, 9662.

Abstract

The UNUSUAL FLORAL ORGANS (UFO) gene is an essential regulatory factor of class B genes and plays a vital role in the process of inflorescence primordial and flower primordial development. We investigated the role of UFO genes in soybean to better understand the development of floral organs through gene cloning, expression analysis, and gene knockout. In situ hybridization demonstrated predominant expression of the GmUFO1 gene in the flower primordium. Phenotypic observation of GmUFO1 knockout mutant lines (Gmufo1) showed obvious alteration in floral organ number and shape, and mosaic organ formation. In contrast, GmUFO2 knockout mutant lines (Gmufo2) showed no obvious difference in floral organs. However, the GmUFO1 and GmUFO2 double knockout lines (Gmufo1ufo2) showed a more severe mutant phenotype than the Gmufo1 lines. Gene expression analysis also showed differences in the expression of major ABC function genes in the knockout lines. Based on the phenotypic and expression analysis, our results suggest the major role of GmUFO1 in the regulation of flower organ formation in soybean and that GmUFO2 does not have any direct effect, but it might have an interaction role with GmUFO1 in the regulation of flower development. In conclusion, the present study identified UFO genes in soybean and improved our understanding of floral development that could be useful for flower design in hybrid soybean breeding.

Keywords

GmUFOs; knockout; floral organ number; floral organ shape; soybean

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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