Stanton, C.R.; Petrovski, S.; Batinovic, S. Isolation of a PRD1-like Phage Uncovers the Carriage of Three Putative Conjugative Plasmids in Clinical Burkholderia Contaminans. Research in Microbiology 2024, 104202, doi:10.1016/j.resmic.2024.104202.
Stanton, C.R.; Petrovski, S.; Batinovic, S. Isolation of a PRD1-like Phage Uncovers the Carriage of Three Putative Conjugative Plasmids in Clinical Burkholderia Contaminans. Research in Microbiology 2024, 104202, doi:10.1016/j.resmic.2024.104202.
Stanton, C.R.; Petrovski, S.; Batinovic, S. Isolation of a PRD1-like Phage Uncovers the Carriage of Three Putative Conjugative Plasmids in Clinical Burkholderia Contaminans. Research in Microbiology 2024, 104202, doi:10.1016/j.resmic.2024.104202.
Stanton, C.R.; Petrovski, S.; Batinovic, S. Isolation of a PRD1-like Phage Uncovers the Carriage of Three Putative Conjugative Plasmids in Clinical Burkholderia Contaminans. Research in Microbiology 2024, 104202, doi:10.1016/j.resmic.2024.104202.
Abstract
The Burkholderia cepacia complex (Bcc) are a group of increasingly multi-drug resistant opportunistic bacteria that can cause severe pulmonary infections. This resistance is driven through a combination of intrinsic factors and the carriage of a broad range of conjugative plasmids harbouring virulence determinants. Therefore, novel treatments are required to not only treat Bcc infection but also to prevent further spread of these virulence determinants. In the search for phages infective for two clinical Bcc isolates, CSP1 phage, a PRD1-like phage was isolated. CSP1 phage was found to require pilus machinery commonly encoded on conjugative plasmids to facilitate infection of multiple Gram-negative bacteria genera including Escherichia and Pseudomonas. Whole genome sequencing and characterisation of one of the clinical Burkholderia isolates revealed it to be Burkholderia contaminans. B. contaminans 5080 was found to contain a genome of over 8 Mbp encoding multiple intrinsic resistance factors, such as efflux pump systems, but more interestingly, carried three novel plasmids encoding multiple putative virulence factors for increased host fitness, including antimicrobial resistance. Even though PRD1-like phages are broad host range, their use in novel antimicrobial treatments shouldn’t be dismissed, as the dissemination potential of conjugative plasmids is extensive. Continued survey of clinical bacterial strains is
Biology and Life Sciences, Immunology and Microbiology
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