PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Evidence for Novel Reticular Corpuscle-Connected Filiform Structures Entangled on the Fascia of the Internal Organs of Rats: The Implication for Cell Death
Version 1
: Received: 7 April 2023 / Approved: 10 April 2023 / Online: 10 April 2023 (02:59:00 CEST)
How to cite:
Lee, B.; Lee, J.Y.; Lee, K.; Lee, I.; Kim, H.G.; Lee, K. Evidence for Novel Reticular Corpuscle-Connected Filiform Structures Entangled on the Fascia of the Internal Organs of Rats: The Implication for Cell Death. Preprints2023, 2023040132. https://doi.org/10.20944/preprints202304.0132.v1
Lee, B.; Lee, J.Y.; Lee, K.; Lee, I.; Kim, H.G.; Lee, K. Evidence for Novel Reticular Corpuscle-Connected Filiform Structures Entangled on the Fascia of the Internal Organs of Rats: The Implication for Cell Death. Preprints 2023, 2023040132. https://doi.org/10.20944/preprints202304.0132.v1
Lee, B.; Lee, J.Y.; Lee, K.; Lee, I.; Kim, H.G.; Lee, K. Evidence for Novel Reticular Corpuscle-Connected Filiform Structures Entangled on the Fascia of the Internal Organs of Rats: The Implication for Cell Death. Preprints2023, 2023040132. https://doi.org/10.20944/preprints202304.0132.v1
APA Style
Lee, B., Lee, J.Y., Lee, K., Lee, I., Kim, H.G., & Lee, K. (2023). Evidence for Novel Reticular Corpuscle-Connected Filiform Structures Entangled on the Fascia of the Internal Organs of Rats: The Implication for Cell Death. Preprints. https://doi.org/10.20944/preprints202304.0132.v1
Chicago/Turabian Style
Lee, B., Hoon Gi Kim and Ki-Bog Lee. 2023 "Evidence for Novel Reticular Corpuscle-Connected Filiform Structures Entangled on the Fascia of the Internal Organs of Rats: The Implication for Cell Death" Preprints. https://doi.org/10.20944/preprints202304.0132.v1
Abstract
Accumulating reports have suggested that an inability to clear dead cells is a cause of inflammation and cancer and that the fascia is associated with cancer metastasis. Novel corpuscle-connected filiform structures (CCFSs) entangled on the fascia of the internal organs of rats were selectively visualized with high repeatability (93%) under vital staining with Janus Green B (JGB). Serial sections of CCFSs stained with haematoxylin and eosin and with Mattson Trichrome were microscopically examined. In parallel, the immunohistochemistry with CD31, Lyve 1, scanning electron microscopy, and transmission electron microscopy (TEM) were also used to investigate the novel properties of the CCFSs. All of the CCFSs enclosed JGB-stained granules, which was verified by the impromptu coupling of stereo and light microscopes and fluorescence-activated cell sorting. The functions of the CCFSs were discovered under fluorescence microscopy and confocal laser scanning microscopy to be implicated in cell death: fragmented DNAs and f-actins with extracellular DNAs. The dead cells in the CCFSs were verified by using terminal deoxynucleotidyl transferase dUTP nick end labelling and TEM. Moreover, the CCFS had a relatively-high concentration of calcium, a main element for cell death in tissue, which was measured in 2-dimensionally images using time-of-flight secondary ion mass spectrometry. Taken these results, we report for the first time that novel reticular CCFSs are widely entangled on the fascia of the internal organs of rats with the implication for cell death.
Keywords
Fascia; Cell death; Repeatability; Immunohistochemistry; Bonghan duct and corpuscle; Janus Green B
Subject
Medicine and Pharmacology, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
