Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles

Version 1 : Received: 5 April 2023 / Approved: 6 April 2023 / Online: 6 April 2023 (12:37:55 CEST)

A peer-reviewed article of this Preprint also exists.

Völzke, J.L.; Smatty, S.; Döring, S.; Ewald, S.; Oelze, M.; Fratzke, F.; Flemig, S.; Konthur, Z.; Weller, M.G. Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles. BioTech 2023, 12, 31. Völzke, J.L.; Smatty, S.; Döring, S.; Ewald, S.; Oelze, M.; Fratzke, F.; Flemig, S.; Konthur, Z.; Weller, M.G. Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles. BioTech 2023, 12, 31.

Abstract

Immobilized metal affinity chromatography (IMAC) is a popular and valuable method for the affinity purification of polyhistidine-tagged recombinant proteins. However, it often shows practical limitations, which might require cumbersome optimizations, additional polishing, and enrichment steps. Here, we present functionalized corundum particles for the efficient, economical, and fast purification of recombinant proteins in a column-free format. The corundum surface is first derivatized with the amino silane APTES, then EDTA dianhydride, and subsequently loaded with nickel ions. The Kaiser test, well-known in solid-phase peptide synthesis, was used to monitor amino silanization and the reaction with EDTA dianhydride. In addition, ICP-MS was performed to quantify the metal-binding capacity. His-tagged protein A/G (PAG), mixed with bovine serum albumin (BSA), was used as a test system. The PAG binding capacity was around 3 mg protein per gram of corundum. Cytoplasm obtained from different E. coli strains was examined as an example of a complex matrix. The imidazole concentration was varied in the loading and washing buffers. As expected, higher imidazole concentrations during loading are usually beneficial when higher purities are desired. Even when higher sample volumes, such as one liter, were used, recombinant protein down to a concentration of 1 µg/mL could be isolated selectively. Comparing the corundum material with standard Ni-NTA agarose beads indicated higher purities of proteins isolated using corundum. His6-MBP-mSA2, a fusion protein consisting of monomeric streptavidin and maltose-binding protein in the cytoplasm of E. coli, was purified successfully. To show that this method is also suitable for mammalian cell culture supernatants, purification of the SARS-CoV-2-S-RBD His8 expressed in human Expi293F cells was performed. The material cost of the nickel-loaded corundum material (without regeneration) is estimated to be less than 30 cents for 1 gram of functionalized support or 10 cents per milligram of isolated protein. Another advantage of the novel system is the corundum particles' extremely high physical and chemical stability. The new material should be applicable in small laboratories and large-scale industrial applications. In summary, we could show that this new material is an efficient, robust, and cost-effective purification platform for the purification of His-tagged proteins, even in challenging, complex matrices and large sample volumes of low product concentration.

Keywords

aluminum oxide; sapphire; ethylenediaminetetraacetic acid; nickel chelate; EDTAD; protein affinity enrichment; hexaHis-Tag; his6; 6xHis; his8; bioseparation; IMAC purification; immunocapture; enrichment; affinity chromatography; solid phase; carrier; material; corundum; nickel; recombinant protein; Escherichia coli; bacterial lysates; protein A/G; cytoplasm

Subject

Biology and Life Sciences, Biology and Biotechnology

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