Version 1
: Received: 14 March 2023 / Approved: 15 March 2023 / Online: 15 March 2023 (02:03:17 CET)
How to cite:
Wang, Z.; Shen, W.; Li, Y.; Wang, X.; Jiang, F.; Zhong, X.; Huang, X.; Liu, J. Integrated Metabolomic and Transcriptomic Study of Klebsiella pneumoniae Strain: Possible Inhibitory Mechanism of Rutin on Strain Growth. Preprints2023, 2023030266. https://doi.org/10.20944/preprints202303.0266.v1.
Wang, Z.; Shen, W.; Li, Y.; Wang, X.; Jiang, F.; Zhong, X.; Huang, X.; Liu, J. Integrated Metabolomic and Transcriptomic Study of Klebsiella pneumoniae Strain: Possible Inhibitory Mechanism of Rutin on Strain Growth. Preprints 2023, 2023030266. https://doi.org/10.20944/preprints202303.0266.v1.
Cite as:
Wang, Z.; Shen, W.; Li, Y.; Wang, X.; Jiang, F.; Zhong, X.; Huang, X.; Liu, J. Integrated Metabolomic and Transcriptomic Study of Klebsiella pneumoniae Strain: Possible Inhibitory Mechanism of Rutin on Strain Growth. Preprints2023, 2023030266. https://doi.org/10.20944/preprints202303.0266.v1.
Wang, Z.; Shen, W.; Li, Y.; Wang, X.; Jiang, F.; Zhong, X.; Huang, X.; Liu, J. Integrated Metabolomic and Transcriptomic Study of Klebsiella pneumoniae Strain: Possible Inhibitory Mechanism of Rutin on Strain Growth. Preprints 2023, 2023030266. https://doi.org/10.20944/preprints202303.0266.v1.
Abstract
The emergence of drug resistant pathogenic bacteria is increasingly challenging conventional antibiotics. Plant derived flavonoids are considered as potential alternatives to antibiotics due to their antimicrobial properties. However, the mechanisms by which flavonoids modulate pathogenic microorganisms’ growth are not fully understood. In our previous studies we found that Rutin, a kind of flavonoids showed inhibition against Klebsiella pneumoniae strains. In order to better understand its inhibitory mechanism on strain growth, we used both Rutin and Luteolin as treatments to incubate K. pneumoniae ATCC700603 strain. After incubation for 4 hours, the homogenous and differential effects of two different flavonoids on the growth of K. pneumoniae were evaluated. The integrated metabolomic and transcriptomic analysis was performed. The expression levels of 5,483 genes and the contents of 882 metabolites were measured. The differentially expressed genes (DEGs) and metabolites (DEMs) were screened and analyzed. The correlation between DEGs and DEMs were also studied. Our results showed that Rutin could inhibit the strain growth by changing metabolic pathways and ABC transporters pathways. Our study also revealed FU841_RS17580 and FU841_RS19145 as functional genes that played vital roles in the strain growth.
Keywords
drug resistance; flavonoids; RNA-seq; Klebsiella pneumoniae; Rutin; antibacterial agents
Subject
LIFE SCIENCES, Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
