Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Creation of Stress-Resistant Mutant Forms for Rice Breeding by Irradiating Seeds with Ionizing Radiation and Treating Them with Salinity and Drought Factors

Nurbol Appazov
ORCID logo ,
Anastasiya Kruglyak
* ORCID logo ,
Yulia Aleksiayenak
ORCID logo ,
Kurmanbek Bakiruly
,
Aidos Zhalbyrov
,
Gulsim Baimbetova
,
Zeinolla Yershin
,
Yury Gledenov
,
Aleksandr Doroshkevich
ORCID logo
Version 1 : Received: 13 March 2023 / Approved: 13 March 2023 / Online: 13 March 2023 (10:28:52 CET)

How to cite: Appazov, N.; Kruglyak, A.; Aleksiayenak, Y.; Bakiruly, K.; Zhalbyrov, A.; Baimbetova, G.; Yershin, Z.; Gledenov, Y.; Doroshkevich, A. Creation of Stress-Resistant Mutant Forms for Rice Breeding by Irradiating Seeds with Ionizing Radiation and Treating Them with Salinity and Drought Factors. Preprints 2023, 2023030229. https://doi.org/10.20944/preprints202303.0229.v1 Appazov, N.; Kruglyak, A.; Aleksiayenak, Y.; Bakiruly, K.; Zhalbyrov, A.; Baimbetova, G.; Yershin, Z.; Gledenov, Y.; Doroshkevich, A. Creation of Stress-Resistant Mutant Forms for Rice Breeding by Irradiating Seeds with Ionizing Radiation and Treating Them with Salinity and Drought Factors. Preprints 2023, 2023030229. https://doi.org/10.20944/preprints202303.0229.v1

Abstract

The article presents research results of the γ-ray and fast neutrons impact on various rice species, in order to obtain mutant forms resistant to salinity (NaCl) and drought factors (sorbitol. They are going to be used as initial forms in synthetic selection when creating varieties, adapted to the stressful conditions of, both, soil and climate in the Kazakhstan Aral Sea. The average lethal doses (LD50) of γ-rays and fast neutrons, as well as the average NaCl and sorbitol lethal concentrations were established. Such environment is best suited for clear manifestation of the mutagenic effect of ionizing radiation and the resistance of rice plants to salinity and drought. A distinct dependence in the effect of ionizing radiation and stress factors on the number of induced resistant mutant forms has been related to the initial rice sort. The largest number of mutant forms was obtained from the local variety Syr Suluy, followed by Leader and AyKerim varieties. From all varieties, the number of mutants obtained from exposure to γ-rays was 43 pieces out of 4500 grains, and from the impact of fast neutrons - 115 pieces out of 2700 grains. M1 mutant plants significantly differ from the initial forms in terms of morphological features – plant height, panicle length, grain size Most plants are characterized by short growth and even dwarfism (˂80 cm). They are lodging tolerant, have short and highly sterile panicles, indicating that they are mutants and resistant to salinity, drought, or both.

Keywords

rice; variety; selection; mutagenesis; gamma rays; fast neutrons; salinity; drought tolerance

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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