Version 1
: Received: 5 March 2023 / Approved: 13 March 2023 / Online: 13 March 2023 (02:18:52 CET)
How to cite:
Du, Z.; Ye, X.; Fu, H.; Li, J.; Ding, J.; Ren, G.; Zhou, L.; Pi, X.; Miao, J. Effect of Dietary Fiber Alleviate Gut Microbiota and Metabolite Associated with High-Meat Diet in Children. Preprints2023, 2023030205. https://doi.org/10.20944/preprints202303.0205.v1.
Du, Z.; Ye, X.; Fu, H.; Li, J.; Ding, J.; Ren, G.; Zhou, L.; Pi, X.; Miao, J. Effect of Dietary Fiber Alleviate Gut Microbiota and Metabolite Associated with High-Meat Diet in Children. Preprints 2023, 2023030205. https://doi.org/10.20944/preprints202303.0205.v1.
Cite as:
Du, Z.; Ye, X.; Fu, H.; Li, J.; Ding, J.; Ren, G.; Zhou, L.; Pi, X.; Miao, J. Effect of Dietary Fiber Alleviate Gut Microbiota and Metabolite Associated with High-Meat Diet in Children. Preprints2023, 2023030205. https://doi.org/10.20944/preprints202303.0205.v1.
Du, Z.; Ye, X.; Fu, H.; Li, J.; Ding, J.; Ren, G.; Zhou, L.; Pi, X.; Miao, J. Effect of Dietary Fiber Alleviate Gut Microbiota and Metabolite Associated with High-Meat Diet in Children. Preprints 2023, 2023030205. https://doi.org/10.20944/preprints202303.0205.v1.
Abstract
The gut microbiota plays a crucial role in childhood obesity, and diet is a dominating driver. The effects of fructo-oligosaccharides (FOS), as a dietary fiber, on the composition and metabolism of gut microbiota in healthy children was investigated by vitro fermentation system with a reformative YCFA medium (rich in tryptic hydrolysates of meat). The 16S rRNA sequencing technology was utilized to analyze the varieties of gut microbiota. Measurement of short chain fatty acids (SCFAs) and gases were used by the gas chromatograph. Majorbio Cloud Platform and MetOrigin, as the interactive cloud server, perform the microbiota analysis, the metabolic pathway enrichment analysis, the statistical correlations, and biological relationships using network visualization. We found that the FOS group significantly regulated the composition and metabolism of gut microbiota. The co-metabolism network showed that 3 metabolites were related to 6 differential bacteria and 8 metabolism pathways. These findings suggest that dietary fiber could regulate the composition of gut microbiota and its metabolites in a better direction, but when dietary fiber participates in precision nutrition formula, it may be relevant for precision obesity, may help identify windows of opportunity for the dietary intervention of childhood obesity.
Keywords
dietary fiber; childhood obesity; gut microbiota; metabolite; high-meat diet
Subject
MEDICINE & PHARMACOLOGY, Nutrition
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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