Review
Version 2
Preserved in Portico This version is not peer-reviewed
Biocontainment Techniques and Applications for Yeast Biotechnology
Version 1
: Received: 6 March 2023 / Approved: 7 March 2023 / Online: 7 March 2023 (16:03:31 CET)
Version 2 : Received: 26 March 2023 / Approved: 27 March 2023 / Online: 27 March 2023 (14:08:10 CEST)
Version 2 : Received: 26 March 2023 / Approved: 27 March 2023 / Online: 27 March 2023 (14:08:10 CEST)
A peer-reviewed article of this Preprint also exists.
Pavão, G.; Sfalcin, I.; Bonatto, D. Biocontainment Techniques and Applications for Yeast Biotechnology. Fermentation 2023, 9, 341. Pavão, G.; Sfalcin, I.; Bonatto, D. Biocontainment Techniques and Applications for Yeast Biotechnology. Fermentation 2023, 9, 341.
Abstract
Biocontainment techniques for genetically modified yeasts (GMYs) are pivotal due to the importance of these organisms for biotechnological processes and also due to the design of new yeast strains by using synthetic biology tools and technologies. Due to the large genetic modifications that many yeast strains display, it is highly desirable to avoid the leakage of GMY cells into natural environments and, consequently, the spread of synthetic genes and circuits by horizontal or vertical gene transfer mechanisms within the microorganisms. Moreover, it is also desirable to avoid that patented yeast gene technologies spread outside the production facility. In this review, it was evaluated the different biocontainment technologies currently available for GMYs. Interestingly, uniplex-type biocontainment approaches (UTBAs), which relies on nutrient auxotrophies induced by gene mutation or deletion, or the expression of simple kill switches apparatus, are still the major biocontainment approaches still in use with GMY. While bacteria like Escherichia coli account for advanced biocontainment technologies based on synthetic biology and multiplex-type biocontainment approaches (MTBAs), GMYs are distant from this scenario due to many reasons. Thus, a comparison of different UTBAs and MTBAs applied for GMY and genetically engineered microorganisms (GEMs) was made, indicating the major advances of biocontainment techniques for GMYs.
Keywords
Biocontainment; Genetically Engineered Microorganisms; Genetically Modified Yeasts; Synthetic Biology; Gene Circuits; Auxotrophies; Kill Switches
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Commenter: Diego Bonatto
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