Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of Different Altitudes on Essential Oil Composition of Origanum majorana Species

Version 1 : Received: 6 March 2023 / Approved: 7 March 2023 / Online: 7 March 2023 (13:44:15 CET)

How to cite: Karaca Öner, E.; Yeşil, M. Effects of Different Altitudes on Essential Oil Composition of Origanum majorana Species. Preprints 2023, 2023030134. https://doi.org/10.20944/preprints202303.0134.v1 Karaca Öner, E.; Yeşil, M. Effects of Different Altitudes on Essential Oil Composition of Origanum majorana Species. Preprints 2023, 2023030134. https://doi.org/10.20944/preprints202303.0134.v1

Abstract

Altitude is an important ecological factor that significantly affects essential oil content, yield and composition. The Origanum majorana, belonging to Origanum genus of Lamiaceae family, is a species with antibacterial and antiviral effects, widely used as a spice and also used in folk medicine for treatment of diseases such as asthma, indigestion, headache and rheumatism. In this study, conducted to examine the effects of altitude on essential oil content and composition of origanum majorana, plant samples were collected from the southern region of Turkey at the beginning of flowering period from seven different altitudes (700-800 m, 800-900 m, 900-1000 m, 1000-1100 m, 1100-1200 m, 1200-1300 m and 1300-1400 m) at 100 m intervals. The highest percentage of essential oil (6.50%) obtained by hydro-distillation was determined at 700-800 m altitudes. The GC-MS analyses revealed that low altitude affected some essential oil components positively. The linalool ratio, which is the major component of the essential oil of Origanum majorana species, was the highest at 700-800 m (79.84%) altitudes. Borneol (0.97%), linalool oxide (1.28%), trans-linalool oxide (1.47%), caryophyllene (2.78%), a- humulene (0.21%), germacrene-D (0.31%) and bicyclogermacrene (2.64%) components yielded high values at 800-900 m altitudes. Thymol (6.28%) and a-terpineol (1.04%), which have an important place in the essential oil composition, increased at 1100-1200 m altitudes; a- terpinene (0.17%), cis-sabinene hydrate (0.82%), terpinene-4-ol (0.55%) and carvacrol (21.50%) increased at 1300-1400 m altitudes.

Keywords

altitude; components; essential oil; linalool; Origanum majorana

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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