Preprint Essay Version 1 Preserved in Portico This version is not peer-reviewed

Pueraria thomsonii Radix Water Extract Alleviates Type 2 Diabetes Mellitus in db/db Mice through the Integrated Regulation of Metabolism and Gut Microbiota

Jiarong Li
† ,
Yuhui Liu
† ,
Hui Ouyang
,
Yong Sun
ORCID logo ,
Youbao Zhong
,
Lifang Wang
,
Jiaxing Huang
,
Junchang Chen
,
Xiangyan Tan
,
Mingyao Li
,
Yue Hu
,
Hua Zhang
* ,
Ronghua Liu
*
†
Jiarong Li and Yuhui Liu have contributed equally to this work.
Version 1 : Received: 5 March 2023 / Approved: 6 March 2023 / Online: 6 March 2023 (04:55:17 CET)

How to cite: Li, J.; Liu, Y.; Ouyang, H.; Sun, Y.; Zhong, Y.; Wang, L.; Huang, J.; Chen, J.; Tan, X.; Li, M.; Hu, Y.; Zhang, H.; Liu, R. Pueraria thomsonii Radix Water Extract Alleviates Type 2 Diabetes Mellitus in db/db Mice through the Integrated Regulation of Metabolism and Gut Microbiota. Preprints 2023, 2023030090. https://doi.org/10.20944/preprints202303.0090.v1. Li, J.; Liu, Y.; Ouyang, H.; Sun, Y.; Zhong, Y.; Wang, L.; Huang, J.; Chen, J.; Tan, X.; Li, M.; Hu, Y.; Zhang, H.; Liu, R. Pueraria thomsonii Radix Water Extract Alleviates Type 2 Diabetes Mellitus in db/db Mice through the Integrated Regulation of Metabolism and Gut Microbiota. Preprints 2023, 2023030090. https://doi.org/10.20944/preprints202303.0090.v1.

Abstract

Type 2 diabetes mellitus(T2DM) is an increasingly prevalent and serious health problem. Its onset is typically associated with metabolic disorders and disturbances in the gut microbiota. Previous studies have reported the anti-T2DM effects of Pueraria thomsonii Radix as a functional food. However, the mechanism of action is still unknown. In this study, the effects of Pueraria thomsonii Radix water extract(PTR) on db/db mice were evaluated by pharmacology, metabolomics, and 16S rRNA gene sequencing. The results showed that PTR could improve the body weight of mice, reduce fasting blood glucose(FBG), urinary glucose(UGLU), homeostasis model assessment insulin resistance(HOMA-IR), urinary albumin/creatinine ratio(UACR), and reduce pancreatic tissue damage. Metabolomics showed that the Model group produced 109 differential metabolites, of which 74 could be regulated by PTR. 16S rRNA sequencing was done in fecal samples and results showed that PTR could reduce the Firmicutes/Bacteroidetes ratio(F/B) associated with obesity and regulate 3 beneficial bacteria and 1 harmful bacteria. In conclusion, the results showed that PTR could ameliorate T2DM symptoms, metabolic disorder, and gut microbiota imbalance of db/db mice, and it was superior to metformin in some aspects. We suggested for the first time that γ-aminobutyric(GABA) may be involved in the regulation of the microbiota-gut-brain axis(MGB) and thus affect the metabolic disorders associated with T2DM. This study will provide a scientific basis for the development of functional food with PTR.

Keywords

Pueraria thomsonii Radix; type 2 diabetes mellitus; metabolomics; gut microbiota

Subject

MEDICINE & PHARMACOLOGY, Nutrition

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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