Version 1
: Received: 26 February 2023 / Approved: 27 February 2023 / Online: 27 February 2023 (07:46:24 CET)
How to cite:
Shaukat, A.; Shaukat, A. Determination of Uric Acid in Homeopathic Acid Uric Tablet by Colorimetric Method. Preprints2023, 2023020456. https://doi.org/10.20944/preprints202302.0456.v1.
Shaukat, A.; Shaukat, A. Determination of Uric Acid in Homeopathic Acid Uric Tablet by Colorimetric Method. Preprints 2023, 2023020456. https://doi.org/10.20944/preprints202302.0456.v1.
Cite as:
Shaukat, A.; Shaukat, A. Determination of Uric Acid in Homeopathic Acid Uric Tablet by Colorimetric Method. Preprints2023, 2023020456. https://doi.org/10.20944/preprints202302.0456.v1.
Shaukat, A.; Shaukat, A. Determination of Uric Acid in Homeopathic Acid Uric Tablet by Colorimetric Method. Preprints 2023, 2023020456. https://doi.org/10.20944/preprints202302.0456.v1.
Abstract
Uric acid being a diagnostic biomarker of gout and therapeutic agent in homeopathic medicines necessitates to develop simple and specific method for its determination. Therefore, present study describes the development and validation of simple and specific colorimetric method for determination of uric acid in homeopathic tablets. Uric acid upon reaction with acid and reagent mixture produced coloured derivative which was detected at 411 nm. The method was found linear over the whole range investigated with the correlation coefficient (R2) = 0.9975. Beer’s law was obeyed over the concentration ranges from 5.0 µg/mL to 240 µg/mL. The method was found to be reliable (95.7 to 108.2% recovery), repeatable intra-day accuracy (97.01 to 107.4%) and reproducible-inter day accuracy (99.45 to 107.8%) with relative standard deviation less than 5%. The results of the present study indicate the method is easy to perform, specific and suitable to be used for the determination of uric acid in homeopathic Acid Uric Tablet using less expensive derivitization.
Keywords
Homeopathic; Uric acid; Derivitization
Subject
CHEMISTRY, Medicinal Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
