Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Multimodal Radiobioconjugates of Magnetic Nanoparticles Labeled with 44Sc and 47Sc for Theranostic Application

Perihan Unak
* ,
Volkan Yasakçı
ORCID logo ,
Elif Tutun
,
K. Buşra Karatay
ORCID logo ,
Rafał Walczak
,
Kamil Wawrowicz
ORCID logo ,
Kinga Żelechowska-Matysiak
ORCID logo ,
Agnieszka Majkowska-Pilip
ORCID logo ,
Aleksander Bilewicz
* ORCID logo
Version 1 : Received: 23 February 2023 / Approved: 24 February 2023 / Online: 24 February 2023 (04:36:10 CET)

A peer-reviewed article of this Preprint also exists.

Ünak, P.; Yasakçı, V.; Tutun, E.; Karatay, K.B.; Walczak, R.; Wawrowicz, K.; Żelechowska-Matysiak, K.; Majkowska-Pilip, A.; Bilewicz, A. Multimodal Radiobioconjugates of Magnetic Nanoparticles Labeled with 44Sc and 47Sc for Theranostic Application. Pharmaceutics 2023, 15, 850. Ünak, P.; Yasakçı, V.; Tutun, E.; Karatay, K.B.; Walczak, R.; Wawrowicz, K.; Żelechowska-Matysiak, K.; Majkowska-Pilip, A.; Bilewicz, A. Multimodal Radiobioconjugates of Magnetic Nanoparticles Labeled with 44Sc and 47Sc for Theranostic Application. Pharmaceutics 2023, 15, 850.

Abstract

This study was performed to synthesize multimodal radiopharmaceutical designed for the diagnosis and treatment of prostate cancer. To achieve this goal, Super Paramagnetic Iron Oxide (SPIO) nanoparticles were used as a platform for targeting molecule (PSMA-617) and for complexation of two scandium radionuclides, 44Sc for PET imaging and 47Sc for radionuclide therapy. TEM and XPS images showed that the Fe3O4 NPs have a uniform cubic shape and a diameter from 38 to 50 nm. The Fe3O4 core are surrounded by SiO2 and an organic layer. The saturation magnetization of the SPION core was 60 emu/g. However, coating the SPIONs with silica and polyglycerol reduces the magnetization significantly. The obtained bioconjugates were labeled with 44Sc and 47Sc, with a yield higher than 97%. The radiobioconjugate exhibited high affinity and cytotoxicity towards the human prostate cancer LNCaP (PSMA+) cell line, much higher than for PC-3 (PSMA-) cells. High cytotoxicity of the radiobioconjugate was confirmed by radiotoxicity studies on LNCaP 3D-spheroids. In addition, the magnetic properties of the radiobioconjugate should allow for its use in guide drug delivery driven by magnetic field gradient.

Keywords

SPION; multimodal nanoparticles; PET diagnosis; MRI; 44/47Sc; PSMA-617; prostate cancer

Subject

Chemistry and Materials Science, Nanotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.