Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Feeding Spray-Dried Porcine Plasma to Pigs Reduces African Swine Fever Virus Load in Infected Pigs and Delays Virus Transmission. Study 1

Elena Blázquez
,
Joan Pujols
,
Fernando Rodriguez
ORCID logo ,
Joaquim Segalés
ORCID logo ,
Rosa Rosell
,
Joy Campbell
ORCID logo ,
Javier Polo
* ORCID logo
Version 1 : Received: 25 January 2023 / Approved: 31 January 2023 / Online: 31 January 2023 (02:21:18 CET)

A peer-reviewed article of this Preprint also exists.

Blázquez, E.; Pujols, J.; Rodríguez, F.; Segalés, J.; Rosell, R.; Campbell, J.; Polo, J. Feeding Spray-Dried Porcine Plasma to Pigs Reduces African Swine Fever Virus Load in Infected Pigs and Delays Virus Transmission—Study 1. Vaccines 2023, 11, 824. Blázquez, E.; Pujols, J.; Rodríguez, F.; Segalés, J.; Rosell, R.; Campbell, J.; Polo, J. Feeding Spray-Dried Porcine Plasma to Pigs Reduces African Swine Fever Virus Load in Infected Pigs and Delays Virus Transmission—Study 1. Vaccines 2023, 11, 824.

Abstract

The objective of this study was to evaluate the potential benefits of feeding spray-dried porcine plasma (SDPP) to pigs infected with African swine fever virus (ASFV). Two groups of twelve weaned pigs each were fed with CONVENTIONAL or 8% SDPP enriched diets. Two pigs (trojans)/group) were injected intramuscularly with the pandemic ASFV (Georgia 2007/01) and comingled with the rest of the pigs (1:5 trojan:naïve ratio) to simulate a natural route of transmission. Trojans developed ASF and died within the first week after inoculation but contact pigs did not develop ASF, viremia or seroconversion. Therefore, three more trojans per group were introduced to optimize the ASFV transmission (1:2 trojan:naïve ratio). Blood, nasal and rectal swabs were weekly harvested and at end of the study, ASFV-target organs collected. After the second exposure, rectal temperature of conventionally fed contact pigs increased >40.5˚C while fever was delayed in the SDPP contact pigs. Additionally, PCR Ct values in blood, secretions and tissue samples were significantly lower (P<0.05) for CONVENTIONAL compared to SDPP contact pigs. Under these study conditions, contact exposed pigs fed SDPP had delayed ASFV transmission and reduced virus load, likely by enhanced specific T-cell priming after the first ASFV-exposure.

Keywords

African swine fever; ASFV; spray-dried porcine plasma; challenge; nutritional intervention

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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