Laser Therapy Changes the Expression of Matrix Metalloproteinases in Bleomycin-Induced Skin Fibrosis

Anna G. Soboleva; Vladimir V. Sobolev; Mari M. Karapetyan; Alexandre Mezentsev; Olga I. Rud’ko; Evgenia D. Davydova; Julia A. Mogulevtseva; Olga V. Zhukova; Irina M. Korsunskaya

doi:10.20944/preprints202301.0497.v1

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preprints.org > biology and life sciences > immunology and microbiology > doi: 10.20944/preprints202301.0497.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Laser Therapy Changes the Expression of Matrix Metalloproteinases in Bleomycin-Induced Skin Fibrosis

^* ,

Julia A. Mogulevtseva

Olga V. Zhukova

Irina M. Korsunskaya

Version 1 : Received: 17 January 2023 / Approved: 27 January 2023 / Online: 27 January 2023 (07:17:38 CET)

A peer-reviewed article of this Preprint also exists.

Soboleva, A. G.; Sobolev, V. V.; Karapetyan, M. M.; Mezentsev, A.; Rud’ko, O. I.; Davydova, E. D.; Mogulevtseva, J. A.; Zhukova, O. V.; Korsunskaya, I. M. Laser Therapy Changes the Expression of Matrix Metalloproteinases in Bleomycin-Induced Skin Fibrosis. Life, 2023, 13, 810. https://doi.org/10.3390/life13030810. Soboleva, A. G.; Sobolev, V. V.; Karapetyan, M. M.; Mezentsev, A.; Rud’ko, O. I.; Davydova, E. D.; Mogulevtseva, J. A.; Zhukova, O. V.; Korsunskaya, I. M. Laser Therapy Changes the Expression of Matrix Metalloproteinases in Bleomycin-Induced Skin Fibrosis. Life, 2023, 13, 810. https://doi.org/10.3390/life13030810.

Abstract

Matrix metalloproteinases (MMPs) are often considered biomarkers of skin fibrosis. At the early stages of the pathological process, an elevation of their enzymatic activity causes significant changes in the composition of the extracellular matrix. MMPs secreted by immune cells facilitate their migration to the site of damage. Then, the immune cells eliminate the affected cells and biomolecules. Moreover, bidirectional changes in the activity of proteolytic enzymes, including MMPs, accompany wound healing. This study aimed to assess changes in the expression of Mmp2, Mmp3, and Mmp9 after treating the mice with laser therapy using the experimental model of bleomycin-induced skin fibrosis. Using immunohistochemistry, we characterized the histological features of scarred skin. We also analyzed changes in the expression of MMPs using real-time polymerase chain reaction before and after the irradiation with laser. We showed that treatment of the mice with CO2 laser partially normalized the histological features of scarred skin. We also noticed a decrease in the expression of Mmp2, Mmp3 (both p < 0.05), and Mmp9 (p = 0.065) during scar healing. The obtained results suggest that normalization of skin homeostasis requires a control of MMPs activities via induction of their genes.

Keywords

matrix metalloproteinases; skin fibrosis; gene expression; laser therapy

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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