Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biosynthesis of Fatty Acid Derivatives by Recombinant Yarrowia Lipolytica Containing Msexd2 and Msexd3 Desaturase Genes from Manduca Sexta

Jaroslav Hambalko
ORCID logo ,
Peter Gajdoš
,
Jean-Marc Nicaud
,
Rodrigo Ledesma-Amaro
ORCID logo ,
Michal Tupec
,
Iva Pichová
,
Milan Čertík
* ORCID logo
Version 1 : Received: 19 December 2022 / Approved: 23 December 2022 / Online: 23 December 2022 (03:50:22 CET)

A peer-reviewed article of this Preprint also exists.

Hambalko, J.; Gajdoš, P.; Nicaud, J.-M.; Ledesma-Amaro, R.; Tupec, M.; Pichová, I.; Čertík, M. Biosynthesis of Fatty Acid Derivatives by Recombinant Yarrowia lipolytica Containing MsexD2 and MsexD3 Desaturase Genes from Manduca sexta. J. Fungi 2023, 9, 114. Hambalko, J.; Gajdoš, P.; Nicaud, J.-M.; Ledesma-Amaro, R.; Tupec, M.; Pichová, I.; Čertík, M. Biosynthesis of Fatty Acid Derivatives by Recombinant Yarrowia lipolytica Containing MsexD2 and MsexD3 Desaturase Genes from Manduca sexta. J. Fungi 2023, 9, 114.

Abstract

One of the most interesting groups of fatty acid derivates is the group of conjugated fatty acids, from which the most researched are conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA), which are associated with countless health benefits. Sex pheromone mixture of some insect species, including tobacco horn-worm (Manduca sexta), are typical for the production of uncommon C16 long conjugated fatty acids with two and three conjugated double bonds, as opposed to C18 long CLA and CLNA. In this study, M. sexta desaturases MsexD2 and MsexD3 were expressed in multiple strains of Y. lipolytica with different genotypes. Experiments with supplementation of fatty acid methyl esters into the medium resulted in production of novel fatty acids. Using GCxGC-MS 20 new fatty acids with two or three double bonds were identified. Fatty acids with conjugated or isolated double bonds or combination of both were produced in trace amounts. Results of this study prove that Y. lipolytica is capable of synthesizing C16 conjugated fatty acids. Further genetic optimization of the Y. lipolytica genome and optimization of the fermentation process could lead to increased production of novel fatty acid derivatives with biotechnologically interesting properties.

Keywords

Yarrowia; pheromone; desaturase; Manduca; conjugase; metabolic engineering

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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