Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Composition and Patterns of Taxa Assemblages in the Western Channel Assessed by 18S Sequencing, Microscopy and Flow Cytometry

Stern Rowena
* ORCID logo ,
Kathryn Picard
,
Jessica Clarke
ORCID logo ,
Charlotte Walker
,
Claudia Martins
,
Clare Marshall
,
Ana Amorim
ORCID logo ,
Malcolm Woodward
ORCID logo ,
Claire Widdicombe
,
Glen Tarran
ORCID logo ,
Martin Edwards
Version 1 : Received: 21 December 2022 / Approved: 22 December 2022 / Online: 22 December 2022 (11:34:13 CET)

A peer-reviewed article of this Preprint also exists.

Stern, R.; Picard, K.; Clarke, J.; Walker, C.E.; Martins, C.; Marshall, C.; Amorim, A.; Woodward, E.M.S.; Widdicombe, C.; Tarran, G.; Edwards, M. Composition and Patterns of Taxa Assemblages in the Western Channel Assessed by 18S Sequencing, Microscopy and Flow Cytometry. J. Mar. Sci. Eng. 2023, 11, 480. Stern, R.; Picard, K.; Clarke, J.; Walker, C.E.; Martins, C.; Marshall, C.; Amorim, A.; Woodward, E.M.S.; Widdicombe, C.; Tarran, G.; Edwards, M. Composition and Patterns of Taxa Assemblages in the Western Channel Assessed by 18S Sequencing, Microscopy and Flow Cytometry. J. Mar. Sci. Eng. 2023, 11, 480.

Abstract

Plankton monitoring by microscopy offers long-term ecological perspective of plankton com-munities but different detection approaches are biased uniquely. Genetic identification of marine plankton has become standard but is still not used in routine monitoring. This study assessed the diversity of plankton taxa using 18S high throughput sequencing from 2011-2012 from small-volume (~200ml) samples from the Water and Microplankton Sampler (WaMS) deployed on the Continuous Plankton Recorder platform (CPR). The 18S-HTS survey revealed a bias towards heterotrophic taxa, and phototrophs under 10µm within the photosynthetic community. In comparison with phytoplankton microscopic counts from the CPR survey and Western Channel Observatory station L4, only 8-12 taxonomic families were common to all three surveys, with a bias towards larger diatoms and dinoflagellate taxa in microscopy surveys. The WaMS survey detected a contrasting but complementary taxa set to that of microscopic surveys. Additional Quantitative PCR was carried out on the picoeukaryotic pelagophyte, Aureococcus anophagefferens, and the nanoeukaryotic potential harmful algae, Pseudo-nitzschia delicatissima, from 2011-2013. This confirmed the persistence presence of A. anophagefferens in the Western Channel and an elevated abundance of both species in 2011. Species specific seasonality were distinct from those of aggregrate phytoplankton groups.

Keywords

Plankton; Monitoring; Harmful algae; microscopic; genetic; Western Channel

Subject

Environmental and Earth Sciences, Environmental Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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