Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Investigation of GOx Stability in Chitosan Matrix: Application for Enzymatic Electrodes

Version 1 : Received: 7 December 2022 / Approved: 7 December 2022 / Online: 7 December 2022 (10:01:10 CET)

A peer-reviewed article of this Preprint also exists.

Chmayssem, A.; Shalayel, I.; Marinesco, S.; Zebda, A. Investigation of GOx Stability in a Chitosan Matrix: Applications for Enzymatic Electrodes. Sensors 2023, 23, 465. Chmayssem, A.; Shalayel, I.; Marinesco, S.; Zebda, A. Investigation of GOx Stability in a Chitosan Matrix: Applications for Enzymatic Electrodes. Sensors 2023, 23, 465.

Abstract

In this study, we designed a new biosensing membrane for the development of an electrochemical glucose biosensor. To proceed, we used a chitosan-based hydrogel that entraps glucose oxidase enzyme (GOx) and we crosslinked the whole matrix using glutaraldehyde, which is known for its quick and reactive crosslinking behavior. Then, the stability of the designed biosensors was investigated over time according to different storage conditions (in PBS solution at temperatures of 4 °C and 37 °C and in the presence or absence of glucose). In some specific conditions, we found that our biosensor is capable to keep its stability for more than six months of storage. We also included catalase to protect the biosensing membranes from the enzymatic reaction by-products (e.g., hydrogen peroxide). This design protects the biocatalytic activity of GOx and enhances the biosensor lifetime.

Keywords

glucose biosensor; chitosan matrix; screen-printed electrode; Glucose Oxidase (GOx); glutaraldehyde crosslinker

Subject

Chemistry and Materials Science, Electrochemistry

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