Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

New Insights into the Biological Activities of Carboxymethylated

Version 1 : Received: 23 November 2022 / Approved: 25 November 2022 / Online: 25 November 2022 (02:52:37 CET)

How to cite: Pires, M.C.; Andriolo, N.D.G.; Lopes, B.R.P.; Delarmelina, C.; Duarte, M.C.T.; Ruiz, A.L.T.G.; Do Nascimento, V.M.G.; De Toledo, K.A.; Dos Santos, C. New Insights into the Biological Activities of Carboxymethylated. Preprints 2022, 2022110465. https://doi.org/10.20944/preprints202211.0465.v1 Pires, M.C.; Andriolo, N.D.G.; Lopes, B.R.P.; Delarmelina, C.; Duarte, M.C.T.; Ruiz, A.L.T.G.; Do Nascimento, V.M.G.; De Toledo, K.A.; Dos Santos, C. New Insights into the Biological Activities of Carboxymethylated. Preprints 2022, 2022110465. https://doi.org/10.20944/preprints202211.0465.v1

Abstract

Lasiodiplodan, an exocellular Lasiodiplodia theobromae β-glucan exopolysaccharide (LaEPS), has at-tracted interest because of its antioxidant, antimicrobial, anti-inflammatory, and antiproliferative properties. LaEPS carboxymethylation enhances LaEPS water solubility and biological activities. However, carboxymethylated fractions of LaEPS (LaEPS-C): LLaEPS-C (Linear) and BLaEPS-C (Branched) were not widely studied yet. So, LaEPS-C, LLaEPS-C, and BLaEPS-C were assessed for their antioxidant, antimicrobial, antiproliferative, anticoagulant, immunomodulatory, and anti-viral activities. Elementary Chemical Composition Analysis confirmed their structural charac-teristics by Energy Dispersive X-Ray Detector (EDS), Fourier Transform Infrared (FTIR), Nuclear Magnetic Resonance (NMR), and Scanning Electron Microscopy (SEM) techniques. The best DPPH scavenging potential was achieved for LLaEPS-C for concentrations lower than 200mg/mL. LaEPS-C showed moderate antiproliferative activity for the NCI-ADR/RES cell (GI50 65.3 µg/mL), and BLaEPS-C showed weak activity for the K562 cell (GI50 235 µg/mL). LLaEPS-C had a reduced Prothrombin Time (PT) and a procoagulant effect. LLaEPS-C and BLaEPS-C are inducers of pro-inflammatory activity due to their ability to induce TNF-α in human macrophages. LLaEPS-C also showed anti-hRSV, which confirmed the antiviral activity of this molecule.

Keywords

Lasiodiplodan; Anticoagulant; Immunomodulatory Activity; Antiviral activities

Subject

Chemistry and Materials Science, Organic Chemistry

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