Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Relative Contribution of Blood Pressure and Renal Sympa-Thetic Nerve Activity on Proximal Tubular Sodium Reabsorption through NHE3 Actions

Roberto B. Pontes
,
Erika E. Nishi
,
Renato O Crajoinas
ORCID logo ,
Maycon I. O Milanez
,
Adriana C. C. Girardi
,
Ruy R Campos
ORCID logo ,
Cassia T Bergamaschi
*
Version 1 : Received: 18 November 2022 / Approved: 25 November 2022 / Online: 25 November 2022 (02:49:16 CET)

A peer-reviewed article of this Preprint also exists.

Pontes, R.B.; Nishi, E.E.; Crajoinas, R.O.; Milanez, M.I.O.; Girardi, A.C.C.; Campos, R.R.; Bergamaschi, C.T. Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity. Int. J. Mol. Sci. 2023, 24, 349. Pontes, R.B.; Nishi, E.E.; Crajoinas, R.O.; Milanez, M.I.O.; Girardi, A.C.C.; Campos, R.R.; Bergamaschi, C.T. Relative Contribution of Blood Pressure and Renal Sympathetic Nerve Activity to Proximal Tubular Sodium Reabsorption via NHE3 Activity. Int. J. Mol. Sci. 2023, 24, 349.

Abstract

This study aimed to verify the effects of an acute increase in blood pressure (BP) and/or renal sympathetic nerve activity (rSNA) on the renal excretion of sodium and water and its potential effect on the regulation of NHE3 activity. Uninephectomized anesthetized male Wistar rats were divided into three experimental groups: 1) Sham, the rats had their BP and rSNA recorded, and urine was collected for 2 h; 2) bicuculline (Bic) paraventricular nucleus of the hypothalamus (PVN), rat BP, and rSNA were recorded, and urine was collected for 1 h at baseline conditions and 1 h after bicuculline injection into the PVN; 3) RNS + Bicuculine injection into the PVN, BP, and rSNA were recorded, and urine was collected 1h after RNS and 1 h after bicuculline injection into the PVN. Renal nerve stimulation (RNS) decreased sodium and water excretion independent of changes in BP. However, after Bic in the PVN during RNS stimulation, BP and rSNA increased up to 30% and 60%, respectively, increasing diuresis (5-fold) and natriuresis (2.3-fold), accompanied by a significant reduction in the NHE3 activity independent of GFR changes. In conclusion, an acute increase in BP overcomes such effects, generating diuresis, natriuresis, and NHE3 activity inhibition.

Keywords

NHE3; glomerular filtration rate; bicuculline; PVN of the hypothalamus; sympathetic nerve activity.

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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