Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Profiling of G-protein Coupled Receptors in Adipose Tissue and Differentiating Adipocytes Offers a Translational Resource for Obesity/Metabolic Research

Saeed Al Mahri
,
Meshail Okla
ORCID logo ,
Mamoon Rashid
,
Shuja Shafi Malik
ORCID logo ,
Jahangir Iqbal
,
Maria Al Ibrahim
,
Ghida Dairi
,
Amer Mahmood
ORCID logo ,
Manikandan Muthurangan
ORCID logo ,
Ahmed Yaqinuddin
ORCID logo ,
Sameer Mohammad
* ORCID logo
Version 1 : Received: 15 November 2022 / Approved: 16 November 2022 / Online: 16 November 2022 (08:52:57 CET)

A peer-reviewed article of this Preprint also exists.

Al Mahri, S.; Okla, M.; Rashid, M.; Malik, S.S.; Iqbal, J.; Al Ibrahim, M.; Dairi, G.; Mahmood, A.; Muthurangan, M.; Yaqinuddin, A.; Mohammad, S. Profiling of G-protein Coupled Receptors in Adipose Tissue and Differentiating Adipocytes Offers a Translational Resource for Obesity/Metabolic Research. Cells 2023, 12, 377. Al Mahri, S.; Okla, M.; Rashid, M.; Malik, S.S.; Iqbal, J.; Al Ibrahim, M.; Dairi, G.; Mahmood, A.; Muthurangan, M.; Yaqinuddin, A.; Mohammad, S. Profiling of G-protein Coupled Receptors in Adipose Tissue and Differentiating Adipocytes Offers a Translational Resource for Obesity/Metabolic Research. Cells 2023, 12, 377.

Abstract

G protein-coupled receptors (GPCRs) are expressed essentially on all cells, facilitating cellular responses to external stimuli, and are involved in nearly every biological process. Several members of this family play significant roles in the regulation of adipogenesis and adipose me-tabolism. However, the expression and functional significance of a vast number of GPCRs in adipose tissue are unknown. We used a high-throughput RT-PCR panel to determine the expres-sion of the entire repertoire of non-sensory GPCRs in mouse white, and brown adipose tissue and assess changes in their expression during adipogenic differentiation of murine adipocyte cell line, 3T3-L1. In addition, the expression of GPCRs in subcutaneous adipose tissues from lean, obese, and diabetic human subjects was evaluated by re-analyzing RNA-sequencing data. We detected a total of 292 and 271 GPCRs in mouse white and brown adipose tissue, respectively. There is a significant overlap in the expression of GPCRs between the two adipose tissue depots but several GPCRs are specifically expressed in one of the two tissue types. Adipogenic differ-entiation of 3T3-L1 cells had a profound impact on the expression of several GPCRs. RNA se-quencing of subcutaneous adipose from healthy human subjects detected 255 GPCRs and obesity significantly changed the expression of several GPCRs in adipose tissue. Finally, we report sev-eral highly expressed GPCRs with no known role in adipose biology whose expression was sig-nificantly altered during adipogenic differentiation and/or in the diseased human subjects. These GPCRs could play an important role in adipose metabolism and serve as a valuable translational resource for obesity and metabolic research.

Keywords

adipose tissue; adipogenesis; G-protein-coupled receptors; thermogenesis; obesity; metabolic syndrome

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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