Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

Diagnostic Accuracy of Methods for Detection of Antibodies against Type I Interferons in Patients with Endocrine Disorders

Version 1 : Received: 7 November 2022 / Approved: 9 November 2022 / Online: 9 November 2022 (02:49:10 CET)
Version 2 : Received: 21 November 2022 / Approved: 22 November 2022 / Online: 22 November 2022 (02:20:41 CET)

A peer-reviewed article of this Preprint also exists.

Nuralieva, N.; Yukina, M.; Sozaeva, L.; Donnikov, M.; Kovalenko, L.; Troshina, E.; Orlova, E.; Gryadunov, D.; Savvateeva, E.; Dedov, I. Diagnostic Accuracy of Methods for Detection of Antibodies against Type I Interferons in Patients with Endocrine Disorders. J. Pers. Med. 2022, 12, 1948. Nuralieva, N.; Yukina, M.; Sozaeva, L.; Donnikov, M.; Kovalenko, L.; Troshina, E.; Orlova, E.; Gryadunov, D.; Savvateeva, E.; Dedov, I. Diagnostic Accuracy of Methods for Detection of Antibodies against Type I Interferons in Patients with Endocrine Disorders. J. Pers. Med. 2022, 12, 1948.

Abstract

Autoantibodies against type 1 interferons (IFN-I) are highly specific marker for type 1 autoimmune polyglandular syndrome (APS-1). In this study, a comparison of three different me-thods, namely, multiplex microarray-based, cell-based and enzyme-linked immunosorbent assays for detection of antibodies against omega-interferon and alpha2-interferon was carried out. A total of 206 serum samples from adult patients with APS-1, APS-2, isolated autoimmune endocrine pa-thologies, non-autoimmune endocrine disorders and healthy individuals were analyzed. In the APS-1 patient cohort (n=18), there was good agreement between the results of anti- IFN-I antibody tests performed by three methods, with 100% specificity and sensitivity for microarray-based assay. Although only the cell-based assay can determine the neutralizing activity of autoantibodies, the microarray-based assay can serve as a highly specific and sensitive screening test to identify anti- IFN-I antibody positive patients.

Keywords

autoantibodies; type I interferon; interferon-ω; interferon-α2; multiplex assay; protein microarray; cell-based autoantibody assay; ELISA

Subject

Biology and Life Sciences, Endocrinology and Metabolism

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