Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

PCR-based Detection of Typhoidal Salmonella from Human Clinical Samples and Evaluation of Traditional PCR, Nested-PCR and Widal Test in Pune, India

Abas O. Hadi
* ,
Hussein Alburkat
* ORCID logo ,
Taisir Abdulelah Kadhim
,
Ammar M S Al-Helli
,
Mustafa Hamza Ghazi
,
Yasir Abdulameer N. Aldabbagh
,
Reem Numaan Talib
,
Sama Hussein Ali
,
Noor Hameed Imran
,
Ruaa Almohsen
,
Turki Muftin Saad
,
Pallavi Mandave
Version 1 : Received: 31 October 2022 / Approved: 1 November 2022 / Online: 1 November 2022 (12:43:58 CET)

How to cite: Hadi, A.O.; Alburkat, H.; Kadhim, T.A.; Al-Helli, A.M.S.; Ghazi, M.H.; Aldabbagh, Y.A.N.; Talib, R.N.; Ali, S.H.; Imran, N.H.; Almohsen, R.; Saad, T.M.; Mandave, P. PCR-based Detection of Typhoidal Salmonella from Human Clinical Samples and Evaluation of Traditional PCR, Nested-PCR and Widal Test in Pune, India. Preprints 2022, 2022110030. https://doi.org/10.20944/preprints202211.0030.v1 Hadi, A.O.; Alburkat, H.; Kadhim, T.A.; Al-Helli, A.M.S.; Ghazi, M.H.; Aldabbagh, Y.A.N.; Talib, R.N.; Ali, S.H.; Imran, N.H.; Almohsen, R.; Saad, T.M.; Mandave, P. PCR-based Detection of Typhoidal Salmonella from Human Clinical Samples and Evaluation of Traditional PCR, Nested-PCR and Widal Test in Pune, India. Preprints 2022, 2022110030. https://doi.org/10.20944/preprints202211.0030.v1

Abstract

Salmonella is a foodborne pathogen associated with localized outbreaks that potentially pose a huge risk to the public health in some countries such as Africa and India, especially in the regions lacking health care measures. The focus of the present study is the molecular detection method of salmonella. The study was conducted to identify Salmonella strains by PCR targeting flagellin specific gene from suspected patient’s clinical blood samples. Serologically, Widal test results showed that all serum samples except one patient (ID 7) were positive for O antigen. The serum of three patients (ID 4, 5 and 10) were found to be positive for H and O antigens while one patient (ID 9) showed agglutination for antigen H. The PCR approach used in this study was successful for fast and precise detection of clinically related Salmonella Typhi. Seven samples out of 10 were positive for Salmonella Typhi as fragments of 458-497bp were observed on the gel-agarose 0.8% corresponds to flagellin gene of S. Typhi. Relatively, smaller amplicons of 366-343bp were observed utilizing nested PCR as well, which seems more sensitive than the conventional PCR. Therefore, we recommend the PCR approach performed in this study to be used as a fast, cost-effective, and time-consuming tool for diagnostic purposes of Salmonella.

Keywords

PCR; Salmonella Typhi; Widal Test; BHI

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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