Review
Version 1
Preserved in Portico This version is not peer-reviewed
Detection of Fish Pathogens in Freshwater Aquaculture Using eDNA Methods: A Review
Version 1
: Received: 18 October 2022 / Approved: 19 October 2022 / Online: 19 October 2022 (14:45:21 CEST)
A peer-reviewed article of this Preprint also exists.
Bohara, K.; Yadav, A.K.; Joshi, P. Detection of Fish Pathogens in Freshwater Aquaculture Using eDNA Methods. Diversity 2022, 14, 1015. Bohara, K.; Yadav, A.K.; Joshi, P. Detection of Fish Pathogens in Freshwater Aquaculture Using eDNA Methods. Diversity 2022, 14, 1015.
Abstract
Organisms release their nucleic acid in the environment including the DNA and RNA which can be used to detect their presence. eDNA/eRNA techniques are being used in different sectors to identify organisms from soil, water, air, and ice since long. The advancement in technology led to easier detection of different organisms without impacting the environment and the organism itself. These methods are being employed in different areas including surveillance, history, and conservation. eDNA and eRNA methods are being extensively used in aquaculture and fisheries setting to understand the presence of different fish species and pathogens in water. However, there are some challenges associated with the reliability of the results because of the degradation of nucleic acid by several factors. In aquaculture there are several diseases and parasites detected with these methods. In this review we discuss different aquaculture diseases and parasites detected with eDNA/eRNA approach and the fate of these nucleic acids when subjected to different water quality and environmental parameters. This review intends to help the researcher about the potential of eDNA/eRNA based detection of pathogens in aquaculture; this will be useful to predict the potential outbreak before it occurs. Along with that this paper intends to make people understand several factors that degrade and can hamper the detection of these nucleic acids.
Keywords
eDNA; eRNA; fish disease; surveillance; hydrolysis; degradation; qPCR
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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