Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isolation and Identification of Arsenic Hyper-Tolerant and Plant Growth Promoting Bacterium from Soil

Debjani Mandal
ORCID logo ,
Mina Aghababaei
,
Sadhan Kr Das
,
Santanu Majumder
* ,
Debashis Chatterjee
* ,
Abhishek Basu
*
Version 1 : Received: 10 October 2022 / Approved: 13 October 2022 / Online: 13 October 2022 (02:01:52 CEST)

A peer-reviewed article of this Preprint also exists.

Mandal, D.; Aghababaei, M.; Das, S.K.; Majumder, S.; Chatterjee, D.; Basu, A. Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil. Minerals 2022, 12, 1452. Mandal, D.; Aghababaei, M.; Das, S.K.; Majumder, S.; Chatterjee, D.; Basu, A. Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil. Minerals 2022, 12, 1452.

Abstract

The soil and groundwater of Bhagobangola I block of Murshidabad district, West Bengal, India is severely arsenic contaminated. A bacterium was isolated from the garden soil of Mahishasthali village, which could tolerate 36.49 mM arsenic (III), 280.44 mM arsenic (V) and 63 mM chromium (III), which makes it arsenic (III & V) and chromium (III) hyper-tolerant bacterium. The growth pattern of this bacterium does not show much alteration in presence of 10 mM arsenic (III) and chromium (III), emphasizing its resistance to these heavy metals. Scanning electron microscopy depicted the size of this bacterium to be ~1.45 µm. 16S rDNA sequencing followed by subsequent phylogenetic analysis established the identity of this bacterium as Microbacterium paraoxydans. The bacterium is capable of bioremediation of arsenic and showed 30.8% & 22.6%, and 35.2% and 30.5% of bioremediation over a period of 24 and 48 hours in 1 mM and 4 mM arsenite, respectively. Microbacterium paraoxydans also exhibit plant growth promoting properties like nitrogen fixation, phosphate solubilization, indole-3-acetic acid production and production of siderophores. Therefore, the heavy metal resistance, bioremediation potential and plant growth promoting potential of the bacterium could be utilized not only for reduction of arsenic toxicity in soil and groundwater but also for plant growth promotion by using it as a biofertilizer.

Keywords

Arsenic hyper-tolerant bacterium; 16S rDNA sequencing; Microbacterium paraoxydans; Arsenic bioremediation; Plant growth promoting bacterium

Subject

Environmental and Earth Sciences, Environmental Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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