Version 1
: Received: 5 October 2022 / Approved: 7 October 2022 / Online: 7 October 2022 (14:32:52 CEST)
How to cite:
Huang, C.; Chiu, S.; Chao, S.; Liao, H.; Lee, S.; Huang, C.; Cho, D. Use of Electric Cell-Substrate Impedance Sensing to Assess the Cytotoxic Effect of Cannabidiol on Human Oral Epidermoid Carcinoma Cells. Preprints2022, 2022100080. https://doi.org/10.20944/preprints202210.0080.v1
Huang, C.; Chiu, S.; Chao, S.; Liao, H.; Lee, S.; Huang, C.; Cho, D. Use of Electric Cell-Substrate Impedance Sensing to Assess the Cytotoxic Effect of Cannabidiol on Human Oral Epidermoid Carcinoma Cells. Preprints 2022, 2022100080. https://doi.org/10.20944/preprints202210.0080.v1
Huang, C.; Chiu, S.; Chao, S.; Liao, H.; Lee, S.; Huang, C.; Cho, D. Use of Electric Cell-Substrate Impedance Sensing to Assess the Cytotoxic Effect of Cannabidiol on Human Oral Epidermoid Carcinoma Cells. Preprints2022, 2022100080. https://doi.org/10.20944/preprints202210.0080.v1
APA Style
Huang, C., Chiu, S., Chao, S., Liao, H., Lee, S., Huang, C., & Cho, D. (2022). Use of Electric Cell-Substrate Impedance Sensing to Assess the Cytotoxic Effect of Cannabidiol on Human Oral Epidermoid Carcinoma Cells. Preprints. https://doi.org/10.20944/preprints202210.0080.v1
Chicago/Turabian Style
Huang, C., Chun-Chung Huang and Der-Yang Cho. 2022 "Use of Electric Cell-Substrate Impedance Sensing to Assess the Cytotoxic Effect of Cannabidiol on Human Oral Epidermoid Carcinoma Cells" Preprints. https://doi.org/10.20944/preprints202210.0080.v1
Abstract
Cannabidiol (CBD) is an active diterpenoid compound that is extracted from the leaves and stem of Cannabis sativa. Previous studies show that CBD is a non-psychotropic compound with significant anti-cancer effects. This study determines its cytotoxic effect on oral cancer cells and OECM1 cells and compares the outcomes with a chemotherapeutic drug, cisplatin. This study determines the effect of CBD on the viability, apoptosis, morphology and migration of OECM1 cells. Electric cell-substrate impedance sensing (ECIS) is used to measure the change in cell impedance for cells that are treated with a series concentration of CBD for 24 hours. AlamarBlue and annexin V/7-AAD staining assays show that CBD has a cytotoxic effect on cell viability and induces cell apoptosis. ECIS analysis shows that CBD decreases the overall resistance and morphological parameters at 4 kHz in a concentration-dependent manner. There is a significant reduction in the wound-healing recovery rate for cells that are treated with 30 μM CBD. This study demonstrates that ECIS can be used for in vitro screening of anticancer drugs and is more sensitive, functional and comprehensive than traditional biochemical assays. CBD also increases cytotoxicity on cell survival and the migration of oral cancer cells, so it may be a therapeutic drug for oral cancer
Keywords
cannabidiol; electric cell-substrate impedance sensing; oral cancer
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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