Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Suppression of CircTCF4 on the Proliferation and Differentiation of Goat Skeletal Muscle Satellite Cells

Shuailong Zheng
ORCID logo ,
Li Li
ORCID logo ,
Helin Zhou
,
Xujia Zhang
,
Xiaoli Xu
,
Dinghui Dai
,
Siyuan Zhan
ORCID logo ,
Jiaxue Cao
ORCID logo ,
Jiazhong Guo
ORCID logo ,
Tao Zhong
ORCID logo ,
Linjie Wang
ORCID logo ,
Hongping Zhang
* ORCID logo
Version 1 : Received: 26 September 2022 / Approved: 27 September 2022 / Online: 27 September 2022 (09:57:42 CEST)

A peer-reviewed article of this Preprint also exists.

Zheng, S.; Li, L.; Zhou, H.; Zhang, X.; Xu, X.; Dai, D.; Zhan, S.; Cao, J.; Guo, J.; Zhong, T.; Wang, L.; Zhang, H. CircTCF4 Suppresses Proliferation and Differentiation of Goat Skeletal Muscle Satellite Cells Independent from AGO2 Binding. Int. J. Mol. Sci. 2022, 23, 12868. Zheng, S.; Li, L.; Zhou, H.; Zhang, X.; Xu, X.; Dai, D.; Zhan, S.; Cao, J.; Guo, J.; Zhong, T.; Wang, L.; Zhang, H. CircTCF4 Suppresses Proliferation and Differentiation of Goat Skeletal Muscle Satellite Cells Independent from AGO2 Binding. Int. J. Mol. Sci. 2022, 23, 12868.

Abstract

The proliferation and differentiation of mammalian skeletal muscle satellite cells (MuSCs) are highly complicated. Apart from the regulatory signaling cascade driven by the protein-coding genes, non-coding RNAs like microRNAs (miRNA) and circular RNAs (circRNAs) play essential roles in this biological process. However, circRNA functions in MuSCs proliferation and differentiation remain largely to be elucidated. Here, we screened for an exonic circTCF4 based on our previous RNA-Seq data, specifically expressed during the development of the longest dorsal muscle in goats. Subsequently, the circular structure and whole sequence of circTCF4 were verified using Sanger sequencing. Besides, circTCF4 was spatiotemporally expressed in multiple tissues from goats but strikingly enriched in muscles. Furthermore, circTCF4 suppressed MuSCs proliferation and differentiation, independent of AGO2 binding. Finally, we conducted Poly(A) RNA-Seq using cells treated with small interfering RNA targeting circTCF4 and found that circTCF4 would affect multiple signaling pathways, including insulin signaling pathway and AMPK signaling pathway related to muscle differentiation. Our results provide additional solid evidence for circRNA regulating skeletal muscle formation.

Keywords

circRNA; skeletal muscle satellite cells; proliferation; differentiation

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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