Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

TEGDMA-Functionalized Dicalcium Phosphate Dihydrate Resin-Based Composites Prevent Secondary Caries in An In-Vitro Biofilm Model

Version 1 : Received: 21 September 2022 / Approved: 23 September 2022 / Online: 23 September 2022 (03:00:11 CEST)

How to cite: Ionescu, A.C.; Hahnel, S.; Chiari, M.D.; König, A.; Delvecchio, P.; Braga, R.R.; Zambelli, V.; Brambilla, E. TEGDMA-Functionalized Dicalcium Phosphate Dihydrate Resin-Based Composites Prevent Secondary Caries in An In-Vitro Biofilm Model . Preprints 2022, 2022090352 (doi: 10.20944/preprints202209.0352.v1). Ionescu, A.C.; Hahnel, S.; Chiari, M.D.; König, A.; Delvecchio, P.; Braga, R.R.; Zambelli, V.; Brambilla, E. TEGDMA-Functionalized Dicalcium Phosphate Dihydrate Resin-Based Composites Prevent Secondary Caries in An In-Vitro Biofilm Model . Preprints 2022, 2022090352 (doi: 10.20944/preprints202209.0352.v1).

Abstract

This study evaluated the efficacy of experimental TEGDMA-functionalized dicalcium phosphate dihydrate (T-DCPD) filler-based resin-based composites (RBC) in preventing caries lesions around the restoration margins (secondary caries”, SC). Standardized Class-II cavities were made in sound molars having the cervical margin in dentin. Cavities were filled with a commercial resin-modified glass-ionomer cement (RMGIC) or experimental RBCs containing a BisGMA-TEGDMA resin blend and one of the following inorganic fractions: 60 wt.% Ba glass (RBC-0); 40 wt.% Ba glass, 20 wt.% T-DCPD (RBC-20); 20 wt.% Ba glass, 40 wt.% T-DCPD (RBC-40). An open-system bioreactor produced S. mutans biofilm-driven SC. Specimens were scanned using micro-CT to evaluate demineralization depths. Scanning Electron Microscopy and Energy-dispersive X-ray Spectroscopy characterized the specimens’ surfaces, while antimicrobial activity, buffering effect, and ion uptake by the biofilms were also evaluated. ANOVA and Tukey’s test were applied at p<0.05. RBC-0 and RBC-20 showed SC development in dentin, while RBC-40 and RMGIC significantly reduced the lesion depth at the restoration margin (p<0.0001). Initial enamel demineralization could be observed only around RBC-0 and RBC-20 restorations. A direct antibiofilm activity could explain SC reduction by RMGIC, while a buffering effect on biofilm’s acidogenicity explained the behavior of RBC-40. Experimental RBC with CaP-releasing functionalized T-DCPD filler could prevent SC with the same efficacy as F-releasing materials.

Keywords

bioreactor; secondary caries; caries model; DCPD; micro-CT

Subject

MATERIALS SCIENCE, Biomaterials

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