Comparison of Two Bacterial Characterization Techniques for the Genomic Analysis of Aquatic Environments

Roberto Bonelly; Victor V. Calderón; Confesora Pinales; Irene Ortiz; Argenys Ovando; Willy Lara; Santo E. Mateo-Perez; Oscar Cardenas-Alegría; Rommel T. Jucá Ramos; Edian F. Franco; Luis O. Maroto

doi:10.20944/preprints202209.0098.v1

How to cite: Bonelly, R.; Calderón, V.V.; Pinales, C.; Ortiz, I.; Ovando, A.; Lara, W.; Mateo-Perez, S.E.; Cardenas-Alegría, O.; Jucá Ramos, R.T.; Franco, E.F.; Maroto, L.O. Comparison of Two Bacterial Characterization Techniques for the Genomic Analysis of Aquatic Environments. Preprints 2022, 2022090098. https://doi.org/10.20944/preprints202209.0098.v1 Bonelly, R.; Calderón, V.V.; Pinales, C.; Ortiz, I.; Ovando, A.; Lara, W.; Mateo-Perez, S.E.; Cardenas-Alegría, O.; Jucá Ramos, R.T.; Franco, E.F.; Maroto, L.O. Comparison of Two Bacterial Characterization Techniques for the Genomic Analysis of Aquatic Environments. Preprints 2022, 2022090098. https://doi.org/10.20944/preprints202209.0098.v1

Abstract

Our research team compared the performance of matrix-assisted laser desorption/ionization followed by a time of flight (MALDI-TOF) mass spectrometry and genomic DNA extraction followed by sequencing, assembly and alignment for phylogenetic assessment. We performed these comparisons to determine our methodology’s overall efficacy and accuracy for environmental bacteria. In addition, we collected samples from various contaminated rivers in the Dominican Republic. For both methods, we analyzed these results and reported the main differences between each method.

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Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Comparison of Two Bacterial Characterization Techniques for the Genomic Analysis of Aquatic Environments

Abstract

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Subject

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