Version 1
: Received: 5 September 2022 / Approved: 5 September 2022 / Online: 5 September 2022 (09:31:44 CEST)
How to cite:
Kiddane, A. T.; Chamika, W. A. S.; Kang, M.-J.; Patil, M. P.; Chun, B.-S.; Kim, G.-D. Anticancer and Apoptotic Activity in Cervical Adenocarcinoma HeLa using Crude Extract from Spike of Stomopneustes variolaris. Preprints2022, 2022090055. https://doi.org/10.20944/preprints202209.0055.v1
Kiddane, A. T.; Chamika, W. A. S.; Kang, M.-J.; Patil, M. P.; Chun, B.-S.; Kim, G.-D. Anticancer and Apoptotic Activity in Cervical Adenocarcinoma HeLa using Crude Extract from Spike of Stomopneustes variolaris. Preprints 2022, 2022090055. https://doi.org/10.20944/preprints202209.0055.v1
Kiddane, A. T.; Chamika, W. A. S.; Kang, M.-J.; Patil, M. P.; Chun, B.-S.; Kim, G.-D. Anticancer and Apoptotic Activity in Cervical Adenocarcinoma HeLa using Crude Extract from Spike of Stomopneustes variolaris. Preprints2022, 2022090055. https://doi.org/10.20944/preprints202209.0055.v1
APA Style
Kiddane, A. T., Chamika, W. A. S., Kang, M. J., Patil, M. P., Chun, B. S., & Kim, G. D. (2022). Anticancer and Apoptotic Activity in Cervical Adenocarcinoma HeLa using Crude Extract from Spike of Stomopneustes variolaris. Preprints. https://doi.org/10.20944/preprints202209.0055.v1
Chicago/Turabian Style
Kiddane, A. T., Byung-Soo Chun and Gun-Do Kim. 2022 "Anticancer and Apoptotic Activity in Cervical Adenocarcinoma HeLa using Crude Extract from Spike of Stomopneustes variolaris" Preprints. https://doi.org/10.20944/preprints202209.0055.v1
Abstract
Cancer is one of the world's most serious health problems and the top cause of mortality today. S. variolaris is a valuable sea creature that has long been used as a folk medicine to cure and prevent ailments. A subcritical water extraction was used method to obtain crude extract from spikes of S. variolaris. The extract had shown antiproliferative effect in HeLa cells with an IC50 of 723.1 ± 9.73 µg/ml, but no toxicity in HEK293. Westernblot was used to detect protein expression; Bax, caspase-8, and IκBα were increased, whereas IKKα and p-NFκB-65(Ser 536) were downregulated. RNA/mRNA expression was revealed by RT-qPCR, and fold changes of caspase-3, cytochrome-c, Bax, Apaf-1, caspase-9, and Bak genes’ expression were raised. Using gel electrophoresis, the treatment groups showed more DNA fragmentation than the control group. GC-MS was used to identify the components in the crude extract; anticancer activity could be attributed to dodecanoic acid, hexadecanoic acid, and tetradecanoic acid content. In conclusion, these results showed the potential use of the crude extract of a spike from S. variolaris with anticancer activity against cervical cancer.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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