Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Characterization of Polyphenolic Compounds from Bacopa Procumbens and Their Effects on Wound Healing Process

Adriana Martinez-Cuazitl
ORCID logo ,
María del Consuelo Gómez-García
,
Oriana Hidalgo-Alegria
,
Olivia Medel-Flores
,
José Alberto Núñez-Gastélum
,
Eduardo San Martín Martínez
,
Ada María Ríos-Cortés
,
Mario Garcia-Solis
,
David Guillermo Pérez-Ishiwara
*
Version 1 : Received: 30 August 2022 / Approved: 1 September 2022 / Online: 1 September 2022 (15:44:09 CEST)

A peer-reviewed article of this Preprint also exists.

Martínez-Cuazitl, A.; Gómez-García, M.C.; Hidalgo-Alegria, O.; Flores, O.M.; Núñez-Gastélum, J.A.; Martínez, E.S.M.; Ríos-Cortés, A.M.; Garcia-Solis, M.; Pérez-Ishiwara, D.G. Characterization of Polyphenolic Compounds from Bacopa procumbens and Their Effects on Wound-Healing Process. Molecules 2022, 27, 6521. Martínez-Cuazitl, A.; Gómez-García, M.C.; Hidalgo-Alegria, O.; Flores, O.M.; Núñez-Gastélum, J.A.; Martínez, E.S.M.; Ríos-Cortés, A.M.; Garcia-Solis, M.; Pérez-Ishiwara, D.G. Characterization of Polyphenolic Compounds from Bacopa procumbens and Their Effects on Wound-Healing Process. Molecules 2022, 27, 6521.

Abstract

Wounds represents a medical problem that contribute importantly to patient morbidity and to the healthcare costs in several pathologies. In Hidalgo, Mexico, Bacopa procumbens plant has been traditionally used for wound healing care for several generations; in vitro and in vivo experiments were design to evaluate the effects of bioactive compounds obtained from B. procumbens aquoethanolic extract and to determine the key pathways involved in wound regeneration. Bioactive compounds were characterized by HPLC- QTOF-MS and proliferation, migration, adhesion, and differentiation studies were done on NIH/3T3 fibroblasts. Polyphenolic compounds from Bacopa procumbens (PB) regulated proliferation and cell adhesion; enhanced migration reducing the artificial scratch area; and modulated cell differentiation. PB compounds were included in a hydrogel for topical administration on rat excision wound model. Histological, histochemical and mechanical analysis showed that PB treatment accelerates wound closure in at least 48 h; reduce inflammation, increasing cell proliferation and deposition and organization of collagen in earlier times. These changes resulted in the formation of a scar with better tensile properties. Immunohistochemistry and RT-PCR molecular analyses demonstrated that treatment induces: i) overexpression of transforming growth factor beta (TGF-β); and ii) the phosphorylation of Smad 2/3 and ERK1/2, suggesting the central role of some PB to enhance wound healing, modulating TGF-β activation.

Keywords

Bacopa procumbens HPLC characterization; effect of polyphenolic compounds; in vitro and in vivo skin wound; collagen organization

Subject

Chemistry and Materials Science, Medicinal Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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