Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Comprehensive Study of De Novo Mutations on Protein-protein Interaction Interface Provides New Insights in Developmental Delay

Version 1 : Received: 18 August 2022 / Approved: 19 August 2022 / Online: 19 August 2022 (04:50:42 CEST)

How to cite: Maharjan, D.T.; Song, W.; Liu, Z.; Wang, W.; Cai, W.; Chen, J.; Xu, F.; Ying, W.; Lin, G.N. A Comprehensive Study of De Novo Mutations on Protein-protein Interaction Interface Provides New Insights in Developmental Delay. Preprints 2022, 2022080355 (doi: 10.20944/preprints202208.0355.v1). Maharjan, D.T.; Song, W.; Liu, Z.; Wang, W.; Cai, W.; Chen, J.; Xu, F.; Ying, W.; Lin, G.N. A Comprehensive Study of De Novo Mutations on Protein-protein Interaction Interface Provides New Insights in Developmental Delay. Preprints 2022, 2022080355 (doi: 10.20944/preprints202208.0355.v1).

Abstract

Mutations, especially those at the protein-protein interaction (PPI) interface, have been associated with various diseases. Meanwhile, though de novo mutations (DNMs) have been proven important in neuropsychiatric disorders, such as developmental delay (DD), the relationship between PPI interface DMNs and DD has not been well studied. Here we curated developmental delay DNM datasets from the PsyMuKB database and showed that DD patients showed a higher rate and deleteriousness in DNM missense on the PPI interface than sibling control. Next, we identified 302 DD-related PsychiPPIs, defined as PPI harboring a statistically significant number of DNM missenses at their interface, and 42 DD candidate genes from PsychiPPI. We then observed that PsychiPPIs preferentially affected hub proteins in the human protein interactome network. When analyzing DD candidate genes using gene ontology and gene spatio-expression, we found that PsychiPPI genes carrying PPI interface mutations, such as FGFR3 and ALOX5, were enriched in development-related pathways and the development of the neocortex, and cerebellar cortex, suggesting their potential involvement in the etiology of DD. Our results demonstrated that DD patients carried an excess burden of PPI-truncating DNM, which could be used to efficiently search for disease-related genes and mutations in large-scale sequencing studies. In conclusion, our comprehensive study indicated the significant role of PPI interface DNMs in developmental delay pathogenicity.

Keywords

developmental delay; de novo mutation; protein-protein interaction; PPI interface; protein in-teractome; PsymuKB

Subject

MEDICINE & PHARMACOLOGY, Psychiatry & Mental Health studies

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