Titus, A.R.; Madeira, P.P.; Ferreira, L.A.; Belgovskiy, A.I.; Mann, E.K.; Mann, J.A., Jr.; Meyer, W.V.; Smart, A.E.; Uversky, V.N.; Zaslavsky, B.Y. Arrangement of Hydrogen Bonds in Aqueous Solutions of Different Globular Proteins. Int. J. Mol. Sci.2022, 23, 11381.
Titus, A.R.; Madeira, P.P.; Ferreira, L.A.; Belgovskiy, A.I.; Mann, E.K.; Mann, J.A., Jr.; Meyer, W.V.; Smart, A.E.; Uversky, V.N.; Zaslavsky, B.Y. Arrangement of Hydrogen Bonds in Aqueous Solutions of Different Globular Proteins. Int. J. Mol. Sci. 2022, 23, 11381.
Titus, A.R.; Madeira, P.P.; Ferreira, L.A.; Belgovskiy, A.I.; Mann, E.K.; Mann, J.A., Jr.; Meyer, W.V.; Smart, A.E.; Uversky, V.N.; Zaslavsky, B.Y. Arrangement of Hydrogen Bonds in Aqueous Solutions of Different Globular Proteins. Int. J. Mol. Sci.2022, 23, 11381.
Titus, A.R.; Madeira, P.P.; Ferreira, L.A.; Belgovskiy, A.I.; Mann, E.K.; Mann, J.A., Jr.; Meyer, W.V.; Smart, A.E.; Uversky, V.N.; Zaslavsky, B.Y. Arrangement of Hydrogen Bonds in Aqueous Solutions of Different Globular Proteins. Int. J. Mol. Sci. 2022, 23, 11381.
Abstract
This work presents the first evidence that dissolved globular proteins change the arrangement of hydrogen bonds in water, with different proteins showing quantitatively different effects. Using ATR-FTIR (Attenuated Total Reflection – Fourier Transform Infrared) spectroscopic analysis of OH-stretch bands, we obtain quantitative estimates of the relative amounts of the previously reported four subpopulations of water structures coexisting in a variety of aqueous solutions. Where solvatochromic dyes can measure the properties of solutions of non-ionic polymers, the results correlate well with ATR-FTIR measurements. In protein solutions to which solvatochromic dye probes cannot be applied, NMR (Nuclear Magnetic Resonance) spectroscopy was used for the first time to estimate the hydrogen bond donor acidity of water. We found strong correlations between the solvent acidity and arrangement of hydrogen bonds in aqueous solutions for several globular proteins. Even quite similar proteins are found to change water properties in dramatically different ways.
Keywords
Fourier Transform Infrared spectroscopy; water structure; hydrogen bonds; protein solution; solvent properties
Subject
Biology and Life Sciences, Biophysics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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