Basabrain, M.; Zhong, J.; Luo, H.; Liu, J.; Yi, B.; Zaeneldin, A.; Koh, J.; Zou, T.; Zhang, C. Formation of Three-Dimensional Spheres Enhances the Neurogenic Potential of Stem Cells from Apical Papilla. Preprints2022, 2022080122. https://doi.org/10.20944/preprints202208.0122.v1
APA Style
Basabrain, M., Zhong, J., Luo, H., Liu, J., Yi, B., Zaeneldin, A., Koh, J., Zou, T., & Zhang, C. (2022). Formation of Three-Dimensional Spheres Enhances the Neurogenic Potential of Stem Cells from Apical Papilla. Preprints. https://doi.org/10.20944/preprints202208.0122.v1
Chicago/Turabian Style
Basabrain, M., Ting Zou and Chengfei Zhang. 2022 "Formation of Three-Dimensional Spheres Enhances the Neurogenic Potential of Stem Cells from Apical Papilla" Preprints. https://doi.org/10.20944/preprints202208.0122.v1
Abstract
Abstract: Cell-based neural regeneration is challenging due to the difficulty in obtaining sufficient neural stem cells with clinical applicability. SCAPs originating from embryonic neural crest with high neurogenic potential could be a promising cell source for neural regeneration. This study aimed to investigate whether the formation of 3D spheres can promote SCAPs neurogenic potential. Material and methods: 3D SCAPs spheres were first generated in 256-well agarose microtissue mold. The spheres and single cells were individually cultured on collagen I coated μ-Slide for 4 and 7 days. Cell morphological changes, neural marker expression, and neurite outgrowth were evaluated under a confocal microscope. Secretion of BDNF and NGF-β was measured by ELISA kits. Results: Pronounced morphological changes were noticed in a time-dependent manner. The migrating cells’ morphology changed from fibroblast-like cells to neuron-like cells. Compared to the 2D culture, neurite length, number, and the expression of neural markers, including Nestin, β-tubulin III, NeuN, and MAP-2 were significantly increased in the 3D spheres, while the secretion of BDNF and NGF-β was markedly downregulated at day 7. Conclusion: The formation of 3D spheres enhanced the neurogenic potential of SCAPs, suggesting the advantage of using the 3D spheres of SCAPs for the treatment of neural diseases.
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
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