Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat

Version 1 : Received: 28 July 2022 / Approved: 4 August 2022 / Online: 4 August 2022 (03:21:28 CEST)

How to cite: Liu, X.; Wang, H.; He, F.; Du, X.; Ren, M.; Bao, Y. The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat. Preprints 2022, 2022080093 (doi: 10.20944/preprints202208.0093.v1). Liu, X.; Wang, H.; He, F.; Du, X.; Ren, M.; Bao, Y. The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat. Preprints 2022, 2022080093 (doi: 10.20944/preprints202208.0093.v1).

Abstract

Cadmium (Cd) is a toxic non–essential element to plants, and its accumulation in crops has significant adverse effects on human health. The cross–talk between copper (Cu) and Cd has been reported, but the molecular mechanisms remain unknown. Here, the function of wheat Cu transporter 3D (TaCOPT3D) in Cd tolerance was investigated. The TaCOPT3D transcripts significantly accumulated in wheat roots under Cd exposure. To test whether TaCOPT3D was involved in response to Cd stress, overexpressing lines with altered expression of TaCOPT3D transporters were compared to wild–type (WT) plants. The results showed that, under 20 μM Cd treatment, TaCOPT3D–overexpression lines exhibited more biomass and lower Cd accumulation in roots, shoots, and grains compared to WT plants. In addition, the TaCOPT3D–overexpression lines demonstrated less reactive oxygen species (ROS) and a greater amount of active antioxidant enzymes under Cd conditions than WT plants. Moreover, TaCOPT3D–overexpression lines highly reduced Cd accumulation under exposure to 20 μM Cu. The regulation pathway of TaCOPT3D in response to Cd stress was investigated, and transcription factor (TF) TaWRKY22, which targeted the TaCOPT3D promoter, was identified. Therefore, TaCOPT3D can serve as a candidate gene for decreasing Cd accumulation in wheat through genetic engineering.

Keywords

wheat; TaCOPT3D; Cd uptake; TaWRKY22; transcriptional regulation

Subject

BIOLOGY, Agricultural Sciences & Agronomy

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.

We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.