Völzke, J.L.; Shamami, P.H.; Gawlitza, K.; Feldmann, I.; Zimathies, A.; Meyer, K.; Weller, M.G. High-Purity Corundum as Support for Affinity Extractions from Complex Samples. Separations2022, 9, 252.
Völzke, J.L.; Shamami, P.H.; Gawlitza, K.; Feldmann, I.; Zimathies, A.; Meyer, K.; Weller, M.G. High-Purity Corundum as Support for Affinity Extractions from Complex Samples. Separations 2022, 9, 252.
Völzke, J.L.; Shamami, P.H.; Gawlitza, K.; Feldmann, I.; Zimathies, A.; Meyer, K.; Weller, M.G. High-Purity Corundum as Support for Affinity Extractions from Complex Samples. Separations2022, 9, 252.
Völzke, J.L.; Shamami, P.H.; Gawlitza, K.; Feldmann, I.; Zimathies, A.; Meyer, K.; Weller, M.G. High-Purity Corundum as Support for Affinity Extractions from Complex Samples. Separations 2022, 9, 252.
Abstract
Nonporous corundum powder, known as an abrasive material in the industry, was functionalized covalently with protein binders to isolate and enrich specific proteins from complex matrices. The materials based on corundum were characterized by TEM, ESEM, BET, DLS, and zeta potential measurements. The strong Al-O-P bonds between the corundum surface and amino phosphonic acids are used to introduce functional groups for further conjugations. The common crosslinker glutaraldehyde was compared with a hyperbranched polyglycerol (PG) of around 10 kDa. The latter is oxidized with periodate to generate aldehyde groups that can covalently react with the amines of the surface and the amino groups from the protein via a reductive amination process. The amount of bound protein was quantified via aromatic amino acid analysis (AAAA). This work shows that oxidized polyglycerol can be used as an alternative to glutaraldehyde. With polyglycerol, more of the model protein bovine serum albumin (BSA) could be attached to the surface under the same conditions, and lower nonspecific binding (NSB) was observed. As a proof of concept, IgG was extracted with protein A from crude human plasma. The purity of the product was examined by SDS-PAGE. A binding capacity of 1.8 mg IgG per g of corundum powder was achieved. The advantages of corundum are the very low price, extremely high physical and chemical stability, pressure resistance, favorable binding kinetics, and flexible application.
Biology and Life Sciences, Biochemistry and Molecular Biology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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