Version 1
: Received: 20 July 2022 / Approved: 22 July 2022 / Online: 22 July 2022 (13:16:10 CEST)
How to cite:
Agbemade, B.; Frempong, T.F.; Badu, A.M. Antioxidant Properties of Sesamum indicum Seeds using Different Solvents. Preprints2022, 2022070340. https://doi.org/10.20944/preprints202207.0340.v1
Agbemade, B.; Frempong, T.F.; Badu, A.M. Antioxidant Properties of Sesamum indicum Seeds using Different Solvents. Preprints 2022, 2022070340. https://doi.org/10.20944/preprints202207.0340.v1
Agbemade, B.; Frempong, T.F.; Badu, A.M. Antioxidant Properties of Sesamum indicum Seeds using Different Solvents. Preprints2022, 2022070340. https://doi.org/10.20944/preprints202207.0340.v1
APA Style
Agbemade, B., Frempong, T.F., & Badu, A.M. (2022). Antioxidant Properties of <em>Sesamum indicum</em> Seeds using Different Solvents. Preprints. https://doi.org/10.20944/preprints202207.0340.v1
Chicago/Turabian Style
Agbemade, B., Theophilus Fadjare Frempong and And Mercy Badu. 2022 "Antioxidant Properties of <em>Sesamum indicum</em> Seeds using Different Solvents" Preprints. https://doi.org/10.20944/preprints202207.0340.v1
Abstract
Sesamum indicum is considered an underutilized oil-bearing seed in the semi-arid regions of Ghana. Nonetheless, it is a promising source of food with both nutritional and therapeutic benefits. The aim of the present study was to evaluate the antioxidant properties S. indicum seeds using different extraction solvents. The seeds were obtained from the local farmers and prepared for analysis. The bioactive compounds present in the seeds were extracted using hexane, ethyl acetate, ethanol and water and their yields quantified. Total phenolic content (TPC), Condensed tannin content (TTC) and Total antioxidant capacity (TAC) , and DPPH radical scaveging assay were analyzed using standard methods. Antinutrients such as saponins, alkaloids, phytates and oxalates were also analyzed from the powdered seeds. Two chemometric methods; hierarchical cluster analysis (HCA) and Pearson correlation were employed to evaluate the interdependence of the various parameters to result in their antioxidant properties. The re-sults revealed that the solvents utilized had a significant impact on the extraction yield, phyto-chemical component concentration, and antioxidant activities. Hexane extracts of S. indicum seeds significantly exhibited the highest antioxidant activity (p < 0.05). It was marked with the highest TAC value of 232.6 ± 6.267 mg/g AAE and a strong DPPH scavenging activity with an IC50 of 52.81 ± 2.30 µg/mL. Correlations (p < 0.05) was established between TPC,CTC, TAC and DPPH radical scavenging activity) of the extracts. Antinutrients such as; phytate, oxalate, saponins and alkaloids were found to be 7.691 ± 0.8576, 1.501 ± 0.1375, 21.33 ± 4.619 and 317.33 ± 30.29 mg/g respectively. Data obtained suggest that S. indicum possess rich bioactive compounds that can be used in neutraceuticals and food products.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
