Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

DEPs Induce Local IgE Class Switching Independent of Their Ability to Stimulate iBALT

Version 1 : Received: 13 July 2022 / Approved: 14 July 2022 / Online: 14 July 2022 (12:16:43 CEST)

How to cite: Chudakov, D.B.; Konovalova, M.V.; Kashirina, E.I.; Kotsareva, O.D.; Shevchenko, M.A.; Tsaregorodtseva, D.S.; Fattakhova, G.V. DEPs Induce Local IgE Class Switching Independent of Their Ability to Stimulate iBALT . Preprints 2022, 2022070222 (doi: 10.20944/preprints202207.0222.v1). Chudakov, D.B.; Konovalova, M.V.; Kashirina, E.I.; Kotsareva, O.D.; Shevchenko, M.A.; Tsaregorodtseva, D.S.; Fattakhova, G.V. DEPs Induce Local IgE Class Switching Independent of Their Ability to Stimulate iBALT . Preprints 2022, 2022070222 (doi: 10.20944/preprints202207.0222.v1).

Abstract

Background. Diesel exhaust particles (DEPs) have a great impact on general increase of atopic diseases worldwide. However, it is still unknown whether DEPs induce systemic B-cell IgE class switching in secondary lymphoid organs or locally in lungs, in inducible bronchial-associated lymphoid tissue (iBALT). The aim of this work was to identify the exact site of DEPs mediated B-cell IgE class switching and pro-allergic antibodies production. Methods. We immunized BALB/c mice with different OVA doses (0.3 and 30 µg) intranasally in the presence and absence of two types of DEPs, SRM1650B and SRM2786. We used low (30 µg) and high (150 µg) DEPs doses in our study. Results. Only high DEP dose induced IgE production regardless of particle type. Local IgE class switching was stimulated upon treatment with both types of particles with both low and high antigen doses. Despite the similar ability of two standard DEP samples to stimulate IgE production, their ability to induce iBALT formation and growing, was markedly different upon co-administration together with low antigen doses. Conclusion. DEPs induced local IgE class switching takes place in pre-existing iBALTs, independently of de novo iBALT formation, at least in the case of SRM1650B co-administrated with low antigen doses.

Keywords

diesel particulate matter; antibody production; tertiary lymphoid structures; local Ig class switch; antigen doses; lungs

Subject

LIFE SCIENCES, Immunology

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