Version 1
: Received: 1 July 2022 / Approved: 7 July 2022 / Online: 7 July 2022 (04:22:14 CEST)
How to cite:
Posukh, O.; Osipov, Y.; Kalashnikova, D.; Antoshina, P.; Belyakin, S.; Singh, P. A Method to Study Extracorporeal Development of Early Planococcus Citri Embryos: a New Schedule for Facultative Heterochromatinization. Preprints2022, 2022070109. https://doi.org/10.20944/preprints202207.0109.v1
Posukh, O.; Osipov, Y.; Kalashnikova, D.; Antoshina, P.; Belyakin, S.; Singh, P. A Method to Study Extracorporeal Development of Early Planococcus Citri Embryos: a New Schedule for Facultative Heterochromatinization. Preprints 2022, 2022070109. https://doi.org/10.20944/preprints202207.0109.v1
Posukh, O.; Osipov, Y.; Kalashnikova, D.; Antoshina, P.; Belyakin, S.; Singh, P. A Method to Study Extracorporeal Development of Early Planococcus Citri Embryos: a New Schedule for Facultative Heterochromatinization. Preprints2022, 2022070109. https://doi.org/10.20944/preprints202207.0109.v1
APA Style
Posukh, O., Osipov, Y., Kalashnikova, D., Antoshina, P., Belyakin, S., & Singh, P. (2022). <strong></strong>A Method to Study Extracorporeal Development of Early <em>Planococcus Citri</em> Embryos: a New Schedule for Facultative Heterochromatinization. Preprints. https://doi.org/10.20944/preprints202207.0109.v1
Chicago/Turabian Style
Posukh, O., Stepan Belyakin and Prim Singh. 2022 "<strong></strong>A Method to Study Extracorporeal Development of Early <em>Planococcus Citri</em> Embryos: a New Schedule for Facultative Heterochromatinization" Preprints. https://doi.org/10.20944/preprints202207.0109.v1
Abstract
Study of imprinted heterochromatinization of the paternal chromosome set in male mealy bugs is made difficult because it takes place at the blastula stage within the ovary. We describe here a method that allows for the bulk preparation of staged early embryos that develop normally outside the mother. We define an accessible experimental window encompassing 48 to 72hours post-mating in which regulation of heterochromatinization of the paternal chromosome set can now be investigated outside the confines of the ovary.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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