Preprint Technical Note Version 1 Preserved in Portico This version is not peer-reviewed

A Method to Study Extracorporeal Development of Early Planococcus Citri Embryos: a New Schedule for Facultative Heterochromatinization

Version 1 : Received: 1 July 2022 / Approved: 7 July 2022 / Online: 7 July 2022 (04:22:14 CEST)

How to cite: Posukh, O.; Osipov, Y.; Kalashnikova, D.; Antoshina, P.; Belyakin, S.; Singh, P. A Method to Study Extracorporeal Development of Early Planococcus Citri Embryos: a New Schedule for Facultative Heterochromatinization. Preprints 2022, 2022070109 (doi: 10.20944/preprints202207.0109.v1). Posukh, O.; Osipov, Y.; Kalashnikova, D.; Antoshina, P.; Belyakin, S.; Singh, P. A Method to Study Extracorporeal Development of Early Planococcus Citri Embryos: a New Schedule for Facultative Heterochromatinization. Preprints 2022, 2022070109 (doi: 10.20944/preprints202207.0109.v1).

Abstract

Study of imprinted heterochromatinization of the paternal chromosome set in male mealy bugs is made difficult because it takes place at the blastula stage within the ovary. We describe here a method that allows for the bulk preparation of staged early embryos that develop normally outside the mother. We define an accessible experimental window encompassing 48 to 72hours post-mating in which regulation of heterochromatinization of the paternal chromosome set can now be investigated outside the confines of the ovary.

Keywords

Heterochromatin; mealy bugs; parent-of-origin effects; genomic imprinting

Subject

BIOLOGY, Animal Sciences & Zoology

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