Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity

Version 1 : Received: 1 July 2022 / Approved: 5 July 2022 / Online: 5 July 2022 (07:49:32 CEST)

How to cite: Schwarzer-Sperber, H.S.; Sutter, K.; Müller, K.; Müller, P.; Schwarzer, R. The Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity . Preprints 2022, 2022070065 (doi: 10.20944/preprints202207.0065.v1). Schwarzer-Sperber, H.S.; Sutter, K.; Müller, K.; Müller, P.; Schwarzer, R. The Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity . Preprints 2022, 2022070065 (doi: 10.20944/preprints202207.0065.v1).

Abstract

Mammalian seminal plasma contains a multitude of bioactive components, including lipids, glucose, mineral elements, metabolites, proteins, cytokines and growth factors, with various functions during insemination and fertilization. The seminal plasma protein PDC-109 is one of the major soluble components of the bovine ejaculate and is crucially important for sperm motility, capacitation and acrosome reaction. A hitherto underappreciated function of seminal plasma is its anti-microbial and anti-viral activity, which may limit sexual transmission of infectious diseases during intercourse. We have recently discovered that PDC-109 inhibits the membrane fusion activity of influenza virus particles and significantly impairs viral infections at micromolar concentrations. Here we investigated whether the antiviral activity of PDC-109 is restricted to Influenza or if other mammalian viruses are similarly affected. We focused on Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), the etiological agent of the Coronavirus Disease 19 (COVID-19), thoroughly assessing PDC-109 inhibition with SARS-CoV-2 Spike (S)-pseudotyped reporter virus particles, but also live-virus infections. Consistent with our previous publications we found significant virus inhibition, albeit accompanied by substantial cytotoxicity. Using time-of-addition experiments however, we discovered treatment regimen that enable virus suppression without affecting cell viability. We furthermore demonstrated that PDC-109 is also able to impair infections mediated by the VSV glycoprotein (VSVg) thus indicating a broad pan-antiviral activity against multiple virus species and families.

Keywords

SARS-CoV-2; VSV replicon; PDC-109; Bovine seminal plasma; Fn-type 2 proteins

Subject

BIOLOGY, Other

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