Schwarzer-Sperber, H.S.; Sutter, K.; Müller, K.; Müller, P.; Schwarzer, R. The Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity. Preprints2022, 2022070065. https://doi.org/10.20944/preprints202207.0065.v1
APA Style
Schwarzer-Sperber, H.S., Sutter, K., Müller, K., Müller, P., & Schwarzer, R. (2022). The Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity. Preprints. https://doi.org/10.20944/preprints202207.0065.v1
Chicago/Turabian Style
Schwarzer-Sperber, H.S., Peter Müller and Roland Schwarzer. 2022 "The Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity" Preprints. https://doi.org/10.20944/preprints202207.0065.v1
Abstract
Mammalian seminal plasma contains a multitude of bioactive components, including lipids, glucose, mineral elements, metabolites, proteins, cytokines and growth factors, with various functions during insemination and fertilization. The seminal plasma protein PDC-109 is one of the major soluble components of the bovine ejaculate and is crucially important for sperm motility, capacitation and acrosome reaction. A hitherto underappreciated function of seminal plasma is its anti-microbial and anti-viral activity, which may limit sexual transmission of infectious diseases during intercourse. We have recently discovered that PDC-109 inhibits the membrane fusion activity of influenza virus particles and significantly impairs viral infections at micromolar concentrations. Here we investigated whether the antiviral activity of PDC-109 is restricted to Influenza or if other mammalian viruses are similarly affected. We focused on Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), the etiological agent of the Coronavirus Disease 19 (COVID-19), thoroughly assessing PDC-109 inhibition with SARS-CoV-2 Spike (S)-pseudotyped reporter virus particles, but also live-virus infections. Consistent with our previous publications we found significant virus inhibition, albeit accompanied by substantial cytotoxicity. Using time-of-addition experiments however, we discovered treatment regimen that enable virus suppression without affecting cell viability. We furthermore demonstrated that PDC-109 is also able to impair infections mediated by the VSV glycoprotein (VSVg) thus indicating a broad pan-antiviral activity against multiple virus species and families.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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