Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Highly Virulent and Multidrug-Resistant Escherichia Coli Sequence Type 58 from a Sausage in Germany

Elias Eger
ORCID logo ,
Marielle Domke
,
Stefan E. Heiden
ORCID logo ,
Madeleine Paditz
,
Veronika Balau
,
Christiane Huxdorff
,
Dirk Zimmermann
,
Timo Homeier-Bachmann
ORCID logo ,
Katharina Schaufler
*
Version 1 : Received: 25 June 2022 / Approved: 28 June 2022 / Online: 28 June 2022 (04:18:22 CEST)

A peer-reviewed article of this Preprint also exists.

Eger, E.; Domke, M.; Heiden, S.E.; Paditz, M.; Balau, V.; Huxdorff, C.; Zimmermann, D.; Homeier-Bachmann, T.; Schaufler, K. Highly Virulent and Multidrug-Resistant Escherichia coli Sequence Type 58 from a Sausage in Germany. Antibiotics 2022, 11, 1006. Eger, E.; Domke, M.; Heiden, S.E.; Paditz, M.; Balau, V.; Huxdorff, C.; Zimmermann, D.; Homeier-Bachmann, T.; Schaufler, K. Highly Virulent and Multidrug-Resistant Escherichia coli Sequence Type 58 from a Sausage in Germany. Antibiotics 2022, 11, 1006.

Abstract

Studies have previously described the occurrence of multidrug-resistant (MDR) Escherichia coli in human and veterinary medical settings, livestock, and, to a lesser extent, in the environment and food. While they mostly analyzed foodborne E. coli regarding phenotypic and sometimes genotypic antibiotic resistance and basic phylogenetic classification, we have limited understanding of the in vitro and in vivo virulence characteristics and global phylogenetic contexts of these bacteria. Here, we investigated an E. coli strain (PBIO3502) isolated from a pork sausage in Germany in 2021 in-depth. Whole-genome sequence analysis revealed sequence type (ST)58, which is an emerging international high-risk clonal lineage. In addition to its MDR phenotype that mostly matched the genotype, PBIO3502 demonstrated pronounced virulence features, including in vitro biofilm formation, siderophore secretion, serum resilience, and in vivo mortality in Galleria mellonella larvae. Along with the genomic analysis indicating close phylogenetic relatedness of our strain with publicly available, clinically relevant representatives of the same ST, these results suggest the zoonotic and pathogenic character of PBIO3502 with the potential to cause infection in humans and animals. Also, our study highlights the necessity of the One Health approach while integrating human, animal, and environmental health, as well as the role of meat products and food chains in the putative transmission of MDR pathogens.

Keywords

antimicrobial resistance; CTX-M-1; Enterobactericeae,; Escherichia coli; extended-spectrum β-lactamase; food safety; IncI1; next-generation sequencing; One Health; virulence

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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