PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
High Pyrethroid Resistance to Deltamethrin and DDT in Major Malaria Vector Anopheles gambiae s.l. from South-Western Nigeria is Probably Driven by Metabolic Resistance Mechanisms
Version 1
: Received: 14 June 2022 / Approved: 17 June 2022 / Online: 17 June 2022 (03:31:08 CEST)
How to cite:
Adeogun, A.O.; Omotayo, A.; Babalola, A.; Joseph, T.; Adesalu, O.; Jimoh, R.; Oyeniyi, T.; Awolola, S.; Ladokun, O. High Pyrethroid Resistance to Deltamethrin and DDT in Major Malaria Vector Anopheles gambiae s.l. from South-Western Nigeria is Probably Driven by Metabolic Resistance Mechanisms. Preprints2022, 2022060243. https://doi.org/10.20944/preprints202206.0243.v1
Adeogun, A.O.; Omotayo, A.; Babalola, A.; Joseph, T.; Adesalu, O.; Jimoh, R.; Oyeniyi, T.; Awolola, S.; Ladokun, O. High Pyrethroid Resistance to Deltamethrin and DDT in Major Malaria Vector Anopheles gambiae s.l. from South-Western Nigeria is Probably Driven by Metabolic Resistance Mechanisms. Preprints 2022, 2022060243. https://doi.org/10.20944/preprints202206.0243.v1
Adeogun, A.O.; Omotayo, A.; Babalola, A.; Joseph, T.; Adesalu, O.; Jimoh, R.; Oyeniyi, T.; Awolola, S.; Ladokun, O. High Pyrethroid Resistance to Deltamethrin and DDT in Major Malaria Vector Anopheles gambiae s.l. from South-Western Nigeria is Probably Driven by Metabolic Resistance Mechanisms. Preprints2022, 2022060243. https://doi.org/10.20944/preprints202206.0243.v1
APA Style
Adeogun, A.O., Omotayo, A., Babalola, A., Joseph, T., Adesalu, O., Jimoh, R., Oyeniyi, T., Awolola, S., & Ladokun, O. (2022). High Pyrethroid Resistance to Deltamethrin and DDT in Major Malaria Vector Anopheles gambiae s.l. from South-Western Nigeria is Probably Driven by Metabolic Resistance Mechanisms. Preprints. https://doi.org/10.20944/preprints202206.0243.v1
Chicago/Turabian Style
Adeogun, A.O., Samson Awolola and Olusola Ladokun. 2022 "High Pyrethroid Resistance to Deltamethrin and DDT in Major Malaria Vector Anopheles gambiae s.l. from South-Western Nigeria is Probably Driven by Metabolic Resistance Mechanisms" Preprints. https://doi.org/10.20944/preprints202206.0243.v1
Abstract
Background: Insecticide resistance in Anopheles gambiae s.l. is a major challenge for malaria vector control in Nigeria. Both target-site insensitivity and metabolic resistance have been im-plicated in resistance process, with the latter receiving little attention in Nigeria. Therefore, we investigated metabolic enzyme activities in Anopheles gambiae s.l populations resistant to Del-tamethrin and Diethyldichlorotriethylethane (DDT) in South-West Nigeria. Methods: Anopheles larvae were collected from Ibadan, Oyo and Badagry, Lagos. Adults were exposed to Deltamethrin and DDT using WHO method. Cohorts of populations were further exposed to Pyperonil Butoxide (PBO) and Deltamethrin. Insecticide-exposed and unexposed co-horts were examined for metabolic enzyme activities. Results were compared between exposed and unexposed samples ANOVA (P<0.05). Results: Mosquitoes were identified as An. gambiae (89%, Ibadan; 0%, Badagry) and An. coluzzii (11%, Ibadan; 100%, Badagry). The populations showed varied level of resistance to Deltamethrin (26%, Ibadan; 71%, Badagry) and DDT (2%, Ibadan; 44%, Badagry). Mortality to Deltamethrin in-creased from 26% to 64% (Ibadan) and 71% to 84% (Badagry) when populations were pre-exposed to PBO. Biochemical analysis revealed significant high levels (P<0.05) of cytochrome P450 and GST in exposed samples. Conclusions: Cytochrome P450 and GST are involved in Deltamethrin and DDT resistance in Anopheles gambiae s.l populations in South-West Nigeria.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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