Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Inactivation Protocols for African Swine Fever Virus in Serum and Saliva Samples

Version 1 : Received: 27 May 2022 / Approved: 30 May 2022 / Online: 30 May 2022 (11:22:19 CEST)

A peer-reviewed article of this Preprint also exists.

Franco-Martínez, L.; Beer, M.; Martínez-Subiela, S.; García-Manzanilla, E.; Blome, S.; Carrau, T. Impact of ASFV Detergent Inactivation on Biomarkers in Serum and Saliva Samples. Pathogens 2022, 11, 750. Franco-Martínez, L.; Beer, M.; Martínez-Subiela, S.; García-Manzanilla, E.; Blome, S.; Carrau, T. Impact of ASFV Detergent Inactivation on Biomarkers in Serum and Saliva Samples. Pathogens 2022, 11, 750.

DOI: 10.3390/pathogens11070750

Abstract

African swine fever (ASF) is a notifiable viral disease of domestic and wild suids. Despite intensive re-search efforts, the pathogenesis of the disease is still far from being understood. Analysis of biomarkers in different body fluids may supplement traditional pathogenesis studies. As reliable protocols are often es-tablished in laboratories with lower biosafety, reliable inactivation of samples is crucial. The objective of this study was to find a procedure that inactivates the virus while preserving the biomarkers for down-stream analyses. To this means, three different inactivation protocols were employed, namely Tergitol-type NP-40 (NP-40) and polyoxyethylene-p-t-octylphenol (Triton X-100), respectively, and one with 95 °C heating. It could be demonstrated that all samples treated with 0.5% (v/v) concentration of both deter-gents showed absence of virus infectivity. The same was true for heated samples. However, heated serum was not suitable for analyses. Next, the treatment impact on biomarker readouts was assessed. While all protocols had an impact on the detection of biomarkers, correlation was retained. Especially NP-40 could be the desired detergent for more accurate measurements while achieving efficient virus inactivation. Based on these studies, samples can be reliably inactivated for most biomarker analyses and thus broader interdisciplinary cooperation is possible.

Keywords

African swine fever; pathogenesis; biomarkers; serum; saliva; virus inactivation; detergent treatment; heat treatment; impact of treatment on biomarkers

Subject

LIFE SCIENCES, Virology

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