Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-induced Apoptosis of Lymphatic Endothelial Cells in vitro

Carina Hromada
ORCID logo ,
Jaana Hartmann
,
Johannes Oesterreicher
,
Anton Stoiber
,
Anna Daerr
,
Barbara Schädl
ORCID logo ,
Eleni Priglinger
,
Andreas H. Teuschl-Woller
,
Wolfgang Holnthoner
ORCID logo ,
Johannes Heinzel
* ,
David Hercher
* ORCID logo
Version 1 : Received: 20 May 2022 / Approved: 23 May 2022 / Online: 23 May 2022 (11:02:07 CEST)

A peer-reviewed article of this Preprint also exists.

Hromada, C.; Hartmann, J.; Oesterreicher, J.; Stoiber, A.; Daerr, A.; Schädl, B.; Priglinger, E.; Teuschl-Woller, A. H.; Holnthoner, W.; Heinzel, J.; et al. Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro. Biomolecules, 2022, 12, 820. https://doi.org/10.3390/biom12060820. Hromada, C.; Hartmann, J.; Oesterreicher, J.; Stoiber, A.; Daerr, A.; Schädl, B.; Priglinger, E.; Teuschl-Woller, A. H.; Holnthoner, W.; Heinzel, J.; et al. Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro. Biomolecules, 2022, 12, 820. https://doi.org/10.3390/biom12060820.

Abstract

Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in case of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly underinvestigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes do not influence the respective other cell types’ migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels in the presence of supporting cells, whereas SCs seemed to exert anti-lymphangiogenic effects when cultured with LECs alone. Here, we describe for the first time increased lymphangiogenesis after peripheral nerve injury and repair. Furthermore, our findings indicate a potential lymph-repellent property of SCs, thereby providing a possible explanation for the lack of lymphatic vessels in the healthy endoneurium. Our results highlight the importance to elucidate the molecular mechanisms of SC-LEC interaction.

Keywords

peripheral nerve regeneration; lymphangiogenesis; Schwann cells; lymphatic endothelial cells

Subject

Medicine and Pharmacology, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.