Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evolutionary and Gene Expression Analyses Reveal New Insights into the Role of LSU Gene-Family in Plant Responses to Sulfate-Deficiency

Version 1 : Received: 21 April 2022 / Approved: 22 April 2022 / Online: 22 April 2022 (03:32:19 CEST)

A peer-reviewed article of this Preprint also exists.

Uribe, F.; Henríquez-Valencia, C.; Arenas-M, A.; Medina, J.; Vidal, E. A.; Canales, J. Evolutionary and Gene Expression Analyses Reveal New Insights into the Role of LSU Gene-Family in Plant Responses to Sulfate-Deficiency. Plants, 2022, 11, 1526. https://doi.org/10.3390/plants11121526. Uribe, F.; Henríquez-Valencia, C.; Arenas-M, A.; Medina, J.; Vidal, E. A.; Canales, J. Evolutionary and Gene Expression Analyses Reveal New Insights into the Role of LSU Gene-Family in Plant Responses to Sulfate-Deficiency. Plants, 2022, 11, 1526. https://doi.org/10.3390/plants11121526.

Abstract

LSU (RESPONSE TO LOW SULFUR) proteins belong to a plant-specific gene family initially characterized by their strong induction in response to sulfate (S) deficiency. However, little is known about the LSU gene repertoire and evolution of this family in land plants. In this work, a total of 270 LSU family members were identified using 134 land plant species with whole genome sequence available. Phylogenetic analysis revealed that LSU genes belong to a Spermatophyta-specific gene family, and their homologs are distributed in three major groups, two for dicotyledons and one group for monocotyledons. Moreover, we analyzed the expression of LSU genes in one representative species of each phylogenetic group (wheat, tomato and Arabidopsis) and found a conserved response to S-deficiency, suggesting that these genes might play a key role in S stress responses. Accordingly, Arabidopsis lsu2 knockout mutant plants showed increased levels of internal sulfate content and lower levels of expression of different key genes involved in S deficiency and metabolism like SDI2 and APR3. In summary, our results indicate that LSU genes are evolutionarily conserved in angiosperms and that specific members of this family might play an important role regulation of S transport and assimilation.

Keywords

sulfate deficiency; Arabidopsis thaliana; Solanum lycopersicum; Triticum aestivum; LSU; response to low sulfur; abiotic stress; sulfur nutrition

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.