Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Novel Mobile Integrons and Strain-Specific Integrase Genes within Shewanella spp. Unveil Multiple Lateral Genetic Transfer Events within the Genus

Version 1 : Received: 16 April 2022 / Approved: 19 April 2022 / Online: 19 April 2022 (03:52:15 CEST)

A peer-reviewed article of this Preprint also exists.

Ayala Nuñez, T.; Cerbino, G.N.; Rapisardi, M.F.; Quiroga, C.; Centrón, D. Novel Mobile Integrons and Strain-Specific Integrase Genes within Shewanella spp. Unveil Multiple Lateral Genetic Transfer Events within The Genus. Microorganisms 2022, 10, 1102. Ayala Nuñez, T.; Cerbino, G.N.; Rapisardi, M.F.; Quiroga, C.; Centrón, D. Novel Mobile Integrons and Strain-Specific Integrase Genes within Shewanella spp. Unveil Multiple Lateral Genetic Transfer Events within The Genus. Microorganisms 2022, 10, 1102.

DOI: 10.3390/microorganisms10061102

Abstract

Shewanella spp. are gram-negative bacteria that thrive in aquatic niches and also cause infectious diseases as opportunistic pathogens. Chromosomal integrons (CI) and mobile integrons (MI) were previously described in some Shewanella isolates. Here, we evaluated the occurrence of integrase genes, the integron systems and their genetic surroundings in the genus. We identified 22 integrase gene types, 17 of which were newly described, in different Shewanella species, evidencing multiple gain and loss events. Phylogenetic analysis showed that most of them were strain-specific, except for Shewanella algae, which seem to have co-evolve within the host as typical CIs. Noteworthy, co-existence of up to 5 different integrase genes, as well as their wide dissemination to Alteromonadales, Vibronales, Chromatiales, Oceanospirillales and Enterobacterales was observed. Identification of novel MIs suggests that lateral genetic transfer may have occurred resembling the behavior of class 1 integrons. The constant emergence of determinants associated to antimicrobial resistance worldwide, concomitantly with novel MIs in strains capable to harbor several types of integrons may be an alarming threat for the recruitment of novel antimicrobial resistance gene cassettes in the genus Shewanella, and its evolution towards the multidrug resistance.

Keywords

Shewanella; chromosomal integron; mobile integron; integrase,gene cassette; lateral genetic transfer

Subject

LIFE SCIENCES, Microbiology

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